目的 探讨芪七连方治疗高血压的分子机制。方法 以超高效液相色谱-串联质谱（UHPLC-MS/MS）测定 芪七连方的成分；以 SWISS ADME 和 SWISS Target Prediction 数据库预测其活性成分及靶点；从 DisGeNET、 DrugBank、GeneCards、OMIM、TTD 数据库获取高血压病基因靶点；将活性成分靶点与高血压病基因靶点取交 集；将交集靶点导入 STRING 数据库预测蛋白质-蛋白质相互作用（PPI）网络，再使用 Cytoscape 3.9.1 的 cytoHubba 插件 MCC 模式取前 5 位作为核心基因；用 Metascape 数据库进行 GO 分析和 KEGG 通路富集分析； 选取核心活性成分与核心基因进行分子对接验证结合活性。 结果 共获得芪七连方 65 种活性成分和 803 个靶 点，1 304 个高血压基因靶点。芪七连方的核心成分为木兰花碱、柚皮素、槲皮素-3-甲基醚、异鼠李素，其 治疗高血压的核心靶点是 STAT3、PTPN11、JAK1 等；芪七连方通过脂质与动脉粥样硬化、PI3K/Akt 信号通路、 AGE-RAGE 信号通路等信号通路调控高血压，分子对接结果显示核心成分和核心靶点的结合能≤-5.0 kcal·mol-1 ， 表明结合效果良好。结论 芪七连方通过多成分、多靶点、多通路防治高血压。

Objective To investigate the molecular mechanism of Qiqilian Formula in treating hypertension. Methods UHPLC-MS/MS was employed to identify the components of Qiqilian Formula. Active components and their corresponding targets were predicted using the SWISS ADME and SWISS Target Prediction databases. Hypertensionrelated genes were obtained from the DisGeNET，DrugBank，GeneCards，OMIM，and TTD databases. Intersecting targets were derived by matching the predicted active component targets with hypertension-related gene targets. These intersecting targets were imported into the STRING database to predict the protein-protein interaction （PPI） network， and the cytoHubba plugin （MCC mode） in Cytoscape 3.9.1 was used to select the top 5 genes as core genes. GO and KEGG pathway enrichment analyses were performed using the Metascape database. Molecular docking was conducted between the core active components and core genes. Results A total of 65 active components and 803 corresponding targets were identified for Qiqilian Formula， along with 1 304 hypertension-related gene targets. The core active components of Qiqilian Formula were magnoflorine， naringenin， quercetin-3-methyl ether， and isorhamnetin. The core targets for treating hypertension included STAT3， PTPN11， and JAK1. Qiqilian Formula appears to regulate hypertension through pathways such as lipid and atherosclerosis， the PI3K/Akt signaling pathway， and the AGERAGE signaling pathway. Molecular docking results indicated favorable binding interactions between the core components and core targets， with binding energies≤-5.0 kcal·mol-1 . Conclusion Qiqilian Formula exerts its preventive and therapeutic effects on hypertension through a multi-component， multi-target， and multi-pathway mechanism.

R285.5

国家自然科学基金资助项目（82360913）。