目的 基于超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS/MS）分析及网络药理学探讨肠炎 宁片治疗溃疡性结肠炎（UC）的药效物质基础及作用机制。方法 采用 UPLC-Q-TOF-MS/MS 技术鉴定肠炎宁 片的主要化学成分及大鼠口服后的入血成分。利用 SwissTargetPrediction 数据库预测入血原型成分的作用靶点； 通过 GeneCards、OMIM 数据库检索 UC 疾病相关靶点；对成分预测靶点与疾病相关靶点取交集，得到肠炎宁 片治疗 UC 的潜在效应靶点。运用 Cytoscape 3.8.0 软件构建“中药-入血原型成分-靶点”相互作用网络，筛选 核心活性成分；将潜在效应靶点导入 STRING 数据库构建蛋白互作（PPI）网络，筛选核心靶点；应用 OmicShare 在线平台对潜在效应靶点进行 GO 功能及 KEGG 通路富集分析；采用 AutoDock Vina 1.2.0 软件对核心活性成分 与核心靶点进行分子对接验证。结果 从肠炎宁片中共鉴定出 73 个化学成分，包括 22 个黄酮类、27 个有机 酸类、8 个环烯醚萜类、4 个苯丙素类、2 个鞣质及 10 个其他类成分。从大鼠血浆中共鉴定出 34 个入血成分， 其中 14 个为原型成分，20 个为代谢产物。共筛选出 149 个潜在效应靶点；6 个核心成分：槲皮素、异鼠李素、 山柰酚、七叶内酯、咖啡酸、没食子酸甲酯；5 个核心靶点：SRC、STAT3、PIK3CA、PIK3R1 及 PTK2。潜在 效应靶点主要富集在 EGFR 酪氨酸激酶抑制剂耐药性、内分泌抵抗、HIF-1、PI3K-Akt、ErbB 等信号通路。分 子对接结果显示入血核心成分与核心靶点具有较好的结合能力。结论 肠炎宁片可能通过槲皮素、异鼠李素、 山柰酚等关键成分，作用于 SRC、STAT3、PIK3CA 等核心靶点，调控 HIF-1、PI3K/AKT 等关键通路，发挥治 疗 UC 的作用。

Objective To investigate the pharmacodynamic material basis and mechanism of action of Changyanning Tablets （CYN） in the treatment of ulcerative colitis （UC） based on ultra performance liquid chromato graphyquadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS/MS） analysis and network pharmacology. Methods The main chemical components of CYN and the constituents absorbed into the bloodstream （blood-derived components） after oral administration in rats were identified using UPLC-Q-TOF-MS/MS. The SwissTargetPrediction database was used to predict the targets of the parent compounds absorbed into the bloodstream；disease-related targets for UC were retrieved from the GeneCards and OMIM databases. The intersection between the component-predicted targets and the disease-related targets was obtained，yielding potential therapeutic targets of CYN for UC. Cytoscape 3.8.0 software was used to construct a "herbal medicine-blood-derived parent components-targets" interaction network to screen core active ingredients. The potential therapeutic targets were imported into the STRING database to construct a proteinprotein interaction （PPI） network for screening core targets. The OmicShare online platform was used for Gene Ontology （GO） function and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analyses of the potential therapeutic targets. Molecular docking verification between the core active ingredients and the core targets was performed using AutoDock Vina 1.2.0 software. Results A total of 73 chemical components were identified from CYN，including 22 flavonoids， 27 organic acids， 8 iridoids， 4 phenylpropanoids， 2 tannins， and 10 other types of components. Thirty-four blood-derived components were identified from rat plasma，of which 14 were parent compounds and 20 were metabolites. A total of 149 potential therapeutic targets were screened out. Six core components were identified： quercetin，isorhamnetin，kaempferol，esculetin，caffeic acid，and methyl gallate. Five core targets were identified： SRC，STAT3，PIK3CA，PIK3R1，and PTK2. The potential therapeutic targets were primarily enriched in signaling pathways such as EGFR tyrosine kinase inhibitor resistance，endocrine resistance，HIF-1，PI3K-Akt，and ErbB. Molecular docking results indicated that the core blood-derived components possessed good binding affinity with the core targets. Conclusion CYN likely exerts its therapeutic effects against UC through key components such as quercetin， isorhamnetin，and kaempferol，acting on core targets including SRC，STAT3，and PIK3CA，and modulating critical pathways such as HIF-1 and PI3K/AKT.

R284.1；R285.5

国家中医药管理局项目（5001-12263004）；江西省中医中药科技创新团队发展计划项目（CXTD22001）；“赣鄱英才计划”创新高端人才 项目（gpyc20240059）；江西省中医药中青年骨干人才（第四批）培养计划项目；江西中医药大学创新创业训练项目（202510412260）。