目的 探讨红花提取物（Carthamus tinctorius L. extract，CTLE）对乙醇诱导的酒精性肝病小鼠氧化应激和炎 症水平的影响及其作用机制。方法 SPF 级 C57BL/6 雄性小鼠随机分为 4 组：对照组、模型组、红花提取物低剂 量组（50 mg·kg-1 ）、红花提取物高剂量组（100 mg·kg-1 ）。对照组给予 Lieber-Decarli 液体饲料，其他组给予 LieberDecarli 酒精饲料构建小鼠慢性酒精性肝损伤模型。收集小鼠血清和肝组织，检测小鼠血清生化指标。通过 HE 和 油红 O 染色观察小鼠肝组织病理变化。qRT-PCR 和 Western Blot 法检测 Keap1/Nrf2 和 STAT3/NF-κB 通路相关因 子的 mRNA 和蛋白表达水平。结果 与模型组比较，给药组丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、低密 度脂蛋白胆固醇（LDL-C）、丙二醛（MDA）的水平明显降低（P＜0.05，P＜0.01），而高密度脂蛋白胆固醇（HDL-C）、 超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平明显升高（P＜0.05，P＜0.01），表明红花提取物对小鼠酒精性肝 损伤具有一定的保护和抗氧化作用； HE 染色和油红 O 染色观察到小鼠肝脏病变和脂质沉积有所改善。并且通 过激活 Keap1/Nrf2 通路相关抗氧化因子的 mRNA 和蛋白表达水平，抑制 STAT3/NF-κB 通路及相关炎症因子的 mRNA 和蛋白表达水平（P＜0.05，P＜0.01），从而增强机体的抗氧化和抗炎作用。结论 红花提取物可以通过调节 Keap1/ Nrf2 和 STAT3/NF-κB 信号通路发挥抗氧化应激和抗炎作用，减轻小鼠的酒精性肝损伤，为治疗酒精性肝病和后 续分子机制研究提供新的思路。

Objective To investigate the effects of Carthamus tinctorius L. extract （CTLE） on the levels of oxidative stress and inflammation in mice with ethanol-induced alcoholic liver disease and its mechanism of action. Methods SPF-grade C57BL/6 male mice were randomly divided into four groups：control group，model group，low-CTLE group （50 mg·kg-1 ），and high-CTLE group （100 mg·kg-1 ）. The control group was given Lieber-Decarli liquid diet， and the other groups were given Lieber-Decarli alcohol diet to construct a chronic alcoholic liver injury model in mice. Serum and liver tissues of mice were collected and serum biochemical indexes of mice were detected. HE and oil red O staining were applied to observe pathological changes in mouse liver tissues. Real-time fluorescence quantitative PCR and Western Blot were used to detect the mRNA and protein expression levels of Keap1/Nrf2 and STAT3/NF- κB pathway-related factors. Results Compared with the model group，the ALT，AST，LDL-C，and MDA levels were significantly reduced （P＜0.05，P＜0.01），while the levels of HDL-C，SOD，and GSH were increased dramatically in the administered group （P＜0.05， P＜0.01）， which indicated that CTLE has specific protective and antioxidant effects on alcoholic liver injury in mice. HE staining and oil red O staining showed that the hepatic lesions and lipid deposition of mice were ameliorated. It enhances the antioxidant and anti-inflammatory effects of the body by activating the mRNA and protein expression levels of antioxidant factors related to the Keap1/ Nrf2 pathway and inhibiting the mRNA and protein expression levels of inflammatory factors related to STAT3/ NF- κB pathway （P＜0.05，P＜0.01）. Conclusion It was shown that CTLE could exert anti-oxidative stress and anti-inflammatory effects through regulating Keap1/Nrf2 and STAT3/NF-κB signaling pathways to attenuate alcoholic liver injury in mice. This study may provide a new idea for the treatment of alcoholic liver disease and the subsequent study of molecular mechanisms.

R285.5

国家自然科学地区基金（82160121）