目的 建立高效液相色谱（HPLC）一测多评法同时测定美洲大蠊中尿嘧啶、尿苷、次黄嘌呤、肌苷及鸟 苷 5 种成分的含量。方法 采用 Agilent ZORBAX SB-Aq 色谱柱（250 mm × 4.6 mm，5 µm）；以甲醇-0.01 mol·L-1 磷酸二氢钾溶液为流动相，梯度洗脱；流速：1.0 mL·min-1；柱温：20 ℃；检测波长：260 nm；进样量： 10 µL。计算内参物尿苷与其他成分的相对校正因子（fa/b）。一测多评法测定 10 批美洲大蠊中 5 种成分含量，并 与外标法比较。结果 5 种核苷类在各自范围内线性良好（r＞0.999 5），平均加样回收率 97.0%~100.8%。尿嘧 啶、次黄嘌呤、肌苷、鸟苷的 fa/b 分别是 0.908 0、1.005 3、1.969 5、1.303 4。结论 所建立的方法准确稳定， 可为美洲大蠊药材及提取物的质量控制提供理论参考。

Objective A HPLC- quantitative analysis of multi-components by single marker （QAMS） was established to determine 5 ingredients including uracil， uridine， hypoxanthine， inosine and guanosine in Periplaneta americana. Methods Separation took place on a Agilent ZORBAX SB-Aq column （250 mm×4.6 mm， 5 µm） by gradient elution of methanol- 0.01 mol·L-1 potassium dihydrogen phosphate at 20 ℃ with a flow rate of 1.0 mL·min-1. The detection wavelength was 260 nm and the injection amount was 10 µL. The relative correction factors （fa/b） was calculated for the other four components with uridine as an internal standard. The content of 5 ingredients in 10 batches of Periplaneta americana was determined by QAMS. Results were compared with those of external standard method （ESM）. Results Five nucleosides showed good linear relationships in their own ranges （r＞0.999 5），and the average recoveries ranged from 97.0% to 100.8%. The relative correction factors of uracil， hypoxanthine，inosine and guanosine were 0.908 0，1.005 3，1.969 5 and 1.303 4，respectively. Conclusion The established method is accurate and stable. It can provide theoretical reference for the quality control of Periplaneta americana.

R285.6

国家自然科学基金项目（82104501）；广州市科技局市校（院）联合资助项目（202201020287）；广州中医药大学第一附属医院国家中医药 传承创新中心科研专项项目（2022QN03）；广东省医院药学研究基金（澳美基金，2024A22）。