目的 分析多花黄精水提物成分，并对其体内外抗肿瘤活性进行评价，为进一步开发利用多花黄精提供 理论基础。方法 （1）通过水提法制备多花黄精水提物，利用 UPLC-Q-TOF/MS、苯酚硫酸法等方法分析多花 黄精水提物化学成分，并通过 CCK-8 法检测多花黄精水提物对多种肿瘤细胞增殖抑制活性，采用流式细胞术 检测细胞周期和凋亡情况，用 Western Blot 法检测凋亡相关蛋白 Bcl-2 和 Bax 的表达情况，利用多花黄精水提 物含药血清检测入血成分对细胞增殖抑制活性。（2）构建荷瘤小鼠模型，随机分为模型组（予以生理盐水）、阳性 药环磷酰胺组（50 mg·kg-1）以及多花黄精水提物低、中、高剂量组（55.9、111.8、223.6 mg·kg-1），灌胃治疗 7 d。 治疗期间，观察小鼠体质量及肿瘤体积变化情况。治疗结束后处死小鼠，采用苏木素-伊红（HE）染色观察心、 肝、脾、肺、肾以及肿瘤组织病理学变化；免疫组化（IHC）检测肿瘤组织 Bcl-2 及 Bax 蛋白表达情况。结果 多花黄精水提物多糖含量达到（10.07±1.3）%，黄酮含量为（0.044±0.004）%，并且通过 UPLC-Q-TOF/MS 检测出 39 种成分，包含黄酮类的黄芩素、槲皮素、木犀草素、芦丁，有机酸类的阿魏酸及多酚类的没食子酸等抗肿 瘤成分。多花黄精水提物对 Hela、A549、4T1、B16、MFC 和 HepG2 细胞均有抑制作用（P＜0.001），其中对 B16 细胞抑制作用最为明显，且多花黄精水提物可诱导 B16 细胞周期阻滞于 G0/G1 期（P＜0.001）。流式双染和 Western Blot 结果显示多花黄精水提物明显促进 B16 细胞凋亡，降低 Bcl-2 的表达，且促进 Bax 的表达（P＜ 0.01，P＜0.001）。多花黄精水提物入血成分对 B16 细胞也具有抑制作用（P＜0.001）。此外，多花黄精水提物 体内活性实验结果显示，与模型组比较，不同剂量的多花黄精水提物能够抑制肿瘤生长，诱导肿瘤细胞坏死， 降低 Bcl-2 表达，提高 Bax 的表达。结论 多花黄精水提物成分丰富，含有多种抗肿瘤活性成分，能抑制肿瘤 生长，诱导肿瘤细胞凋亡，显示较强的抗肿瘤作用，值得深入研究。

Objective To analyze the composition of the aqueous extract of Polygonatum Cyrtonema Hua （PCHE） and evaluate its antitumor activity in vitro and in vivo. Our aim is to provide a theoretical basis for the further development and utilization of Polygonatum Cyrtonema Hua. Methods （1） PCHE was prepared by aqueous extraction， and the chemical composition of PCHE was analyzed by UPLC-Q-TOF/MS and phenol-sulfuric acid method. The inhibitory activity on tumor cells proliferation of PCHE was detected by CCK-8 assay. Cell cycle and apoptosis were detected by flow cytometry， and the expression of apoptosis-related proteins Bcl-2 and Bax was detected by Western Blot. The inhibitory activity of PCHE-containing serum on cell proliferation was detected. （2） A B16 tumor-bearing mice model was constructed and model mice were randomly divided into the model group （saline），the positive drug group （CTX：50 mg·kg-1），and PCHE low-，medium-，and high- dose groups （55.9， 111.8，223.6 mg·kg-1），and treated by gavage for 7 days. Changes in body weight and tumor volume of mice were observed during the treatment period. The mice were executed after the treatment，and the histopathological changes of heart，liver，spleen，lung，kidney and tumor were observed by hematoxylin-eosin （HE） staining. The protein expression of Bcl-2 and Bax in tumor tissues was detected by immunohistochemistry （IHC） . Results The polysaccharide content of PCHE reached （10.07±1.3）% ， and the flavonoid content was （0.044±0.004）% ， and thirty-nine components were detected by UPLC-Q-TOF/MS， which contained antitumor components such as flavonoids （baicalein，quercetin，luteolin and rutin），organic acids （ferulic acid）and polyphenols （gallic acid）， etc. PCHE exhibited the inhibitory effects on Hela，A549，4T1，B16，MFC and HepG2 cells，among which the inhibitory effect on B16 cells was the most significant （P＜0.001），and PCHE induced cell cycle arrest at G0/G1 phase in B16 cells （P＜0.001） . The results of double-staining flow cytometry and Western Blot showed that PCHE significantly promoted apoptosis of B16 cells，decreased the expression of Bcl-2，and promoted the expression of Bax （P＜0.01，P＜0.001） . and PCHE constituents absorbed into blood also had an inhibitory effect on B16 cells （P＜0.001） . In addition，the results of in vivo activity assay showed that different doses of PCHE could inhibit tumor growth，induce tumor cell necrosis，reduce Bcl-2 expression，and increase Bax expression compared with the model group. Conclusion The ingredients in PCHE are abundant. It contains a variety of antitumor active ingredients，which can inhibit tumor growth，induce tumor cell apoptosis，show strong anti-tumor effects and be worthy of in-depth study.

R285.5

安徽省教育厅项目自然科学重点项目（KJKJ2020A0628，KJ2021A0951，2022AH051673，2022AH051687）；安徽省大别山中医药研究 院开放课题（TCMADM-2023-04）；安徽省现代中药产业共性技术研究中心开放课题（AHTCMGTRC-2023-03）。