目的 改进和优化清热败毒合剂的制剂提取工艺。方法 采用双提法提取金银花等药物，通过高效液相色谱测定清热败毒合剂中绿原酸的含量。运用SPSS软件进行方差分析，通过对指标成分进行综合评分确定最佳提取工艺。结果 确定其最佳条件为加水量8倍，浸泡60 min，煎煮时间为2 h，煎煮2次。结论 绿原酸含量高于原有的提取工艺，改进的提取工艺优于原有的工艺。

Objective To optimize the extraction process for Qingre Baidu mixture which has the heat-clearing and detoxifying actions. Methods Two extraction methods were used. Firstly，aqueous extraction and steam distillation were used for Flos Lonicerae，and then the content of chlorogenic acid was assayed by HPLC. SPSS software was used for the analysis of variance and the extraction process was optimized after comprehensive analysis of the indicative components. Results The optimized extraction process condition was as follows：soaking the herbs of the decoction with 8 times of water for 60 min，and then decocting for two times，2 hours for each time. Conclusion Higher chlorogenic acid has been obtained through the improved extraction process，indicating that the improved process is superior to the conventional process.

R284.2

上海市卫生局中药新药及院内制剂研发项目（2011ZJ015）；上海市海派中医传承研究项目（ZYSNXD-CC-APGC-JD002）