目的 采用高效液相色谱（HPLC）法建立绿原酸和连翘酯苷A的含量测定方法，并对制备过程中绿原酸和连翘酯苷A含量进行监测，为金英黄归汤制备工艺改进提供质控标准。方法 采用Hypersil C18色谱柱（150 mm × 4.6 mm，5 μm）；绿原酸和连翘酯苷A的流动相分别为乙腈-0.2 %磷酸（11 ∶ 89）与乙腈-0.4 %冰醋酸（17 ∶83）；检测波长分别为327 nm和330 nm；柱温分别为35 ℃和30 ℃；流速分别为0.8 m•min-1和1.0 mL•min-1。结果 绿原酸和连翘酯苷A的线性范围分别为：13.56～217.00 μg•mL-1（r2=0.9997），0.0536～0.2680 mg•mL-1（r2=0.9996）；稳定性试验RSD分别为0.14 %和0.47 %；中间精密度试验RSD分别为0.63 %和0.56 %；重复性试验RSD分别为0.68 %和1.00 %；平均回收率分别为绿原酸99.23 %，RSD 为0.61 %；连翘酯苷A100.24 %，RSD为1.15 %。结论 建立的绿原酸和连翘酯苷A含量检测方法，可为金英黄归汤制备过程提供质控数据。

Objective To establish the determination method for chlorogenic acid and forsythoside A in Jinying Huanggui decoction by HPLC，thus to provide the quality control standard for the improved product technology of Jinying Huanggui decoction. Methods Hypersil C18 column（150 mm× 4.6 mm，5 μm） was used. The mobile phase of chlorogenic acid was acetonitrile-0.2% phosphonic acid solution（11∶89） and that of forsythoside A was acetonitrile -0.4 % glacial acetic acid（17∶83）. The detection wavelength for chlorogenic acid and forsythoside A was set at 327 nm and 330 nm，the column temperature was 35 ℃ and 30 ℃，and the flow rate was 0.8 mL•min-1 and 1.0 mL•min-1，respectively. Results The linear range of chlorogenic acid and forsythoside A in the established method was 13.56～217.00 μg•mL-1（r2=0.9997） and 0.0536～0.2680 mg•mL-1（r2=0.9996）， RSD of stability test was 0.14% and 0.47%，RSD of intermedial precision test was 0.63% and 0.56%，and RSD of the repeatability test was 0.68% and 1.00%，respectively. The average recovery of chlorogenic acid was 99.23%，RSD being 0.61%，and that of forsythoside A was 100.24%，RSD being 1.15%. Conclusion The determination method for chlorogenic acid and forsythoside A has been established，and the method will provide evidence for the quality control of Jinying Huanggui decoction preparation process.

R284.1

国家自然科学基金项目（31060347）。