目的 建立不同产地独活中蛇床子素和欧前胡素含量的测定方法。方法 采用液相色谱-质谱联用（液-质联用）方法，测定4种不同产地独活中蛇床子素和欧前胡素的含量。液相色谱条件：甲醇-水（体积比，85∶15）作流动相，流速为0.3 mL•min-1，柱温30 ℃，进样量5 μL。质谱条件：电喷雾离子源（ESI），阳离子模式，多反应监测（MRM）方式检测。采用外标法测定蛇床子素和欧前胡素的含量。结果 不同产地独活中蛇床子素含量范围为1.11～5.02 μg•g-1，欧前胡素的含量范围0.85～9.54 μg•g-1。结论 不同产地独活中蛇床子素和欧前胡素的含量存在一定的差异。该法可为不同地区独活中药材的质量控制提供参考标准。

Objective To establish a method for the determination of osthole and imperatorin in Radix Angelicae Pubescentis from different habitats. Methods High performance liquid chromatography-mass spectrometry/ mass spectrometry（HPLC-MS/MS） was used for the determination of osthole and imperatorin in Radix Angelicae Pubescentis from four habitats. Chromatographic analysis was carried out on a C18 column（3.5 μm，10 cm×2.1 mm） with methanol-water（v/v being 85∶15） as mobile phase. The flow rate was 0.3 mL•min-1，column temperature was 30 ℃，and injection volume was 5 μL. The condition of mass spectrometry was as follows：electrospray ionization（ESI） in cationic mode，and multiple reaction monitoring（MRM） detection mode. Finally，external standard method was used for the determination of osthole and imperatorin in Radix Angelicae Pubescentis. Results The content of osthole was in the range of 1.11～5.02 μg•g-1 and that of imperatorin was in the range of 0.85～9.54 μg•g-1. Conclusion The contents of osthole and imperatorin in Radix Angelicae Pubescentis from different habitats vary，which may provide the reference standard for quality control of Radix Angelicae Pubescentiss.

R284.1

江西中医药大学校基金——道地药材及特色中药资源研究专项（2012ZR022）。