目的 用近红外光谱技术快速测定国产血竭中龙血素A、龙血素B含量。方法 根据国产血竭在5284～4169.34 cm-1、7131.47～5943.53 cm-1、8913.37～7470.88 cm-1内的近红外吸收光谱，采用偏最小二乘法（PLS）建立了校正模型。采用二阶导数预处理方法时能最有效地提取光谱的信息。结果 龙血素A的校正集相关系数为0.9749，校正集标准偏差（RMSEC）为0.1720，预测集相关系数为0.9857，预测集标准偏差（RMSEP）为0.1450；龙血素B校正集相关系数为0.9933，校正集标准偏差（RMSEC）为0.0526，预测集相关系数为0.9872，预测集标准偏差（RMSEP）为0.0604。结论 近红外光谱技术快速简便，适合中药指标成分的快速分析。

Objective To develop a method for the determination of loureirin A and loureirin B in China domestic Resina Draconis using near infrared diffuse reflectance spectroscopy. Methods Near infrared spectra（NIR） in the range of 5284～4169.34 cm-1，7131.47～5943.53 cm-1，8913.37～7470.88 cm-1 were recorded for Resina Draconis. Calibration model was established using the partial least squares（PLS） method. Spectra pretreatments were extracted by second-order derivate pretreatment method. Results For loureirin A，the correlation coefficient（r2） of calibration set was 0.9749 with RMSEC being 0.1720 and the correlation coefficient（r2） of prediction sets was 0.9857 with RMSEP being 0.1450. For loureirin B，the correlation coefficient（r2） of calibration set was 0.9933 with the root square standard error of calibration（RMSEC） being 0.0526，and the correlation coefficient（r2） of prediction sets was 0.9872 with the root square standard error of prediction being 0.0604. Conclusion It is indicated that near infrared diffuse reflectance spectroscopy can be used to rapidly analyze the valid components in Chinese herbs.

R284.1

北京中医药大学“重点学科”开发课题（2013-2DXKKF-21）。