目的 建立直接数码扫描薄层色谱的影像，利用计算机软件测定黄连提取物中巴马汀、小檗碱、表小檗碱、黄连碱含量的新方法。方法 薄层色谱的制备：硅胶高效薄层板；展开剂为乙酸丁酯∶甲醇∶异丙醇∶氨水（25 %～28 %）=5∶2∶1.5∶1.2；定量操作获得荧光薄层色谱经摄像获取色谱的影像，经专业软件数码扫描，替代常规薄层色谱需采用薄层扫描仪定量的测定方法，利用获取的色谱中小檗碱、巴马汀、表小檗碱、黄连碱斑点的积分值，直接外标法同时测定含量。结果 经精密度、准确度、重复性测试，结果符合方法学验证的技术要求。与液相色谱测定的数据比较完全具有可比性。结论 用自编计算机软件（‘digiscan’）作为虚拟扫描仪进行数码扫描薄层色谱的影像（薄层色谱视觉数据的永久记录），通用软件Origin Pro积分，外标法计算含量，不需要昂贵的薄层色谱扫描仪，即可进行含量测定，通过黄连提取物中4种生物碱的含量测定，证明这一替代方法用于中药有效成分含量测定的可行性。本法最大优点是不需要昂贵的薄层扫描仪，依靠软件操作，薄层色谱的影像可长期保存，测定不受时间和地点的限制，检测成本效益高。符合中药指标成分含量测定的需求，有推广应用的价值，尤其适合生产企业的全程检验、新药研发过程检验及市场商品的检验。

Objective To establish a method of direct digital scanning of thin layer chromatogram integrated with professional software for quantitative determination of four alkaloids（palmatine，berberine，coptisine，epiberberine） in extractive of Rhizoma Coptidis. Methods Thin layer chromatography（TLC） was conducted on pre-coated Silica gel 60 high performance TLC plate，developing with the solvent system of n-butyl acetate，methanol，isopropanol and ammonia（25 %-28 %） in the proportion of 5∶2∶1.5∶1.2. The available TLC image was photographed under visualization device，and the TLC image of the four alkaloids was scanned and analyzed by self-developed professional software“Digiscan” and commercial software“Origin pro”. The contents of four alkaloids of palmatine，berberine，coptisine，epiberberine were also detected by direct external standard method. Results The accuracy，repeatability and reproducibility of the established method were complied with the requirements of the methodology validation. The results were compatible with those of the HPLC. Conclusion Digital scanning of thin layer chromatogram integrated with professional software is feasible for the quantitative determination of four alkaloids（palmatine，berberine，coptisine，epiberberine） in extractive of Rhizoma Coptidis. With home-developed software（‘digiscan’） as virtual scanner and by scoring the peak area and calculating the content by means of 'Origin Pro' software without need of the expensive professional TLC Scanner，the established method shows good prospect for the determination of effective components in Chinese medicine. It can be expected to be used as the alternative method of quantitative TLC analysis for quality control of herbal drugs for it is convenient，economical，practical（it can be performed at anytime and anywhere），and so it would be especially suitable for manufacture process quality control and marketing quality supervising.

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