目的 通过HPLC指纹图谱探索三黄汤的药效物质基础。方法 采用迪马公司Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm），甲醇（A）- 1 ％磷酸水溶液（B）梯度洗脱，流速1.0 mL•min-1，柱温30℃，检测波长277 nm，对10批三黄汤样本进行检测分析，建立三黄汤HPLC指纹图谱，对比相同条件下体外复方图谱、单味药材图谱、药材阴性图谱及混合对照品图谱，初步确认三黄汤主要有效成分及来源。结果 在该色谱条件下，三黄汤指纹图谱中标示出44个共有峰，7个成分得到认定，其中绿原酸来源于茵陈；盐酸小檗碱来源于黄柏；黄芩苷来源于黄芩；甘草酸铵来源于甘草；大黄酸、大黄素、大黄酚来源于大黄。结论 该方法准确可靠，重复性好，为三黄汤有效成分的确定提供了一定的实验依据。

Objective To explore the active components in Sanhuang decoction（SHD） based on the establishment of SHD fingerprint. Methods The method was established on a Diamonsil C18 column（250 mm×4.6 mm，5 μm） with mobile phase composed of methanol（A） -1 % aqueous phosphoric acid（B） in gradient elution. The flow rate was 1.0 mL•min-1. The column temperature was 30℃ and the detection wavelength was 277 nm. By analysis of 10 batches of SHD samples，HPLC fingerprinting was established. The main effective ingredients and their material sources in SHD were identified after comparing the in-vitro fingerprint of SHD under the same conditions，fingerprint of single herb medicine，fingerprint of negative patterns and fingerprint of mixed reference. Results Under the chromatographic conditions，44 common peaks were presented in SHD fingerprint，and 7 components were identified，including chlorogenic acid from Herba Artemisiae Scopariae，berberine from Cortex Phellodendri，baicalin from Radix Scutellariae，ammonium glycyrrhizinate from Radix Glycyrrhizae，and rhein，emodin and chrysophanol from Radix et Rhizoma Rhei. Conclusion The method is accurate and reproducible，which provides experimental evidence for the determination of the effective components of SHD.

辽宁省自然科学基金项目（201102234）。