目的 探讨表没食子儿茶素没食子酸酯［（-）-epigallocatechin-3-gallate，EGCG）］联合MicroRNA-34a对骨肉瘤MG63细胞的增殖抑制作用。方法 将骨肉瘤MG63细胞分成EGCG组、MicroRNA-34a组、联合组和生理盐水组。MTT实验及细胞克隆形成实验研究EGCG联合MicroRNA-34a对MG63细胞的毒性。流式细胞技术研究EGCG联合MicroRNA-34a诱导MG63细胞凋亡作用。构建裸鼠骨肉瘤异位肿瘤模型，研究EGCG联合RNA干预对裸鼠肿瘤的生长抑制能力。结果 与生理盐水组和单独给药组比较，EGCG联合MicroRNA-34a对MG63细胞的生长及克隆形成抑制作用显著强于其他组，差异有统计学意义（P＜0.01）。EGCG联合MicroRNA-34a能促进MG63细胞的凋亡（P＜0.01）。EGCG联合MicroRNA-34a能够显著延长荷瘤小鼠的中位生存期。结论 EGCG联合MicroRNA-34a能够有效抑制骨肉瘤MG63细胞的增殖。

Objective To evaluate the effect of （-） -epigallocatechin-3-gallate（EGCG） combined with MicroRNA-34a on inhibiting the proliferation of osteosarcoma MG63 cells. Methods The MG63 cells were divided into four groups，EGCG group，MicroRNA-34a group，combination group（EGCG +MicroRNA-34a），and saline group. MTT assay was used to evaluate the anti-proliferation efficiency of EGCG combined with MicroRNA-34a. Flow cytometry was used to evaluate the MG63 cells apoptosis induced by EGCG + MicroRNA-34a. Osteosarcoma MG63 cells were xenografted into the mice to establish the animal model，which was used to evaluate the anti-tumor effect. Results The combination group could inhibit the growth of MG63 cells effectively（P＜0.05），promote the apoptosis of MG63 cells，and prolong median survival of the tumor-bearing nude mice compared to saline group，EGCG group and MicroRNA-34a group. Conclusion EGCG combined with MicroRNA-34a can inhibit the proliferation of osteosarcoma MG63 cells.

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