目的 探索香附油滴丸抗痛经作用及其机制。方法 采用小鼠离体子宫平滑肌收缩试验，观察受试药对子宫平滑肌收缩张力及p42/44丝裂原激活蛋白激酶（p42/44MAPK）磷酸化水平和间隙连接蛋白43（Cx43）表达水平的影响；采用缩宫素致小鼠痛经试验，观察受试药的抗痛经作用及对血栓烷B2（TXB2）和6-酮-前列腺素F1α（6-Keto-PGF1α）表达、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活力的影响。结果 受试药连续灌胃给药7 d，显著降低小鼠子宫平滑肌收缩张力及p42/44MAPK磷酸化水平和Cx43表达水平（P＜0.05，P＜0.01）；明显抑制缩宫素致小鼠痛经反应，降低小鼠血浆TXB2水平和血清MDA含量及升高小鼠血浆6-Keto-PGF1α水平和血清SOD活力（P＜0.05，P＜0.01）。结论 香附油滴丸具有明显的抗痛经作用，其作用机制可能与抑制p42/44MAPK磷酸化水平和Cx43表达，调节TXB2/6-Keto-PGF1α平衡及抗氧化作用相关。

Objective To explore the anti-dysmenorrheal effects and mechanism of Xiangfu You Diwan（XYD）. Methods The isolated mouse uterine smooth muscle contraction test was carried out to explore the action of XYD on tension of uterine smooth muscle，p42/44 mitogen-activated protein kinase（p42/44MAPK） activity and connexin 43（Cx43） expression. The oxytocin- induced dysmenorrhea test in mouse model was used to evaluate the anti-dysmenorrheal effect of XYD，the expression levels of thromboxane B2（TXB2） and 6-keto-prostaglandin F1α（6-Keto-PGF1α），malondialdehyde（MDA） content，and superoxide dismutase（SOD）activity in blood. Results XYD significantly decreased the contraction of mouse uterine smooth muscle，p42/44MAPK activity and Cx43 expression after oral administration for 7 days（P＜0.05，P＜0.01），and obviously relieved the oxytocin-induced dysmenorrhea，decreased TXB2 level and MDA content，and increased 6-Keto-PGF1α level and SOD activity in blood（P＜0.05，P＜0.01）. Conclusion XYD has significant anti-dysmenorrheal effects，and its mechanism may be relevant to inhibiting p42/44MAPK activity and Cx43 expression，regulating the balance of TXB2，6-Keto-PGF1α and counteracting oxidation.

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江苏省卫生职业技术教育研究课题（J201215）；江苏省高校“青蓝工程”中青年学术带头人培养对象基金（2012）。