目的 观察中药泽泻中指标成分23-乙酰泽泻醇B对细胞线粒体能量代谢的影响。方法 以SGC-7901胃癌细胞为研究对象，绘制其生长曲线，MTT法检测细胞增殖抑制率，以阳离子荧光羰花青染料JC-1染色，采用倒置荧光显微镜观察结合流式细胞仪检测对细胞线粒体膜电位；多功能酶标仪检测琥珀酸脱氢酶（SDH）活性。结果 23-乙酰泽泻醇B 3.125 μg•mL-1干预能促进细胞增殖，23-乙酰泽泻醇B 25 ~100 μg•mL-1干预则不同程度的抑制细胞增殖，并且呈浓度依赖关系。与正常对照组比较，不同剂量组的红色荧光和绿色荧光的变化不明显。流式细胞仪检测结果显示，正常对照组、23-乙酰泽泻醇B低、中、高剂量组的单阳细胞比例分别为3.5 %，3.6 %，3.7 %和4.0 %，与正常对照组比较，23-乙酰泽泻醇B低剂量组单阳细胞比例增加，但差异无统计学意义（P \> 0.05），23-乙酰泽泻醇B中、高剂量组单阳细胞比例增加，差异有统计学意义（P＜0.05，P＜0.01）；SDH检测结果显示，正常对照组、23-乙酰泽泻醇B低、中、高剂量组的SDH活性分别为0.045、0.058、0.080、0.100 μmol•min-1，与正常对照组比较，23-乙酰泽泻醇B低剂量组的SDH活性差异无统计学意义（P \> 0.05），23-乙酰泽泻醇B中、高剂量组的SDH活性差异有统计学意义（P＜0.05，P＜0.01）。结论 23-乙酰泽泻醇B在低剂量时能促进SGC-7901细胞增殖，中剂量与高剂量则对SGC-7901线粒体膜电位有一定的损伤而抑制增殖，但对SDH活性促进作用较显著，提示其具有较明显促进线粒体能量代谢的作用，为进一步探讨泽泻改善线粒体能量代谢物质基础的研究奠定了基础。

Objective To observe the effect of alisol B 23-acetate（ABA），an effective component of Rhizoma Alismae，on mitochondrial energy metabolism of SGC-7901 cells. Methods Human gastric carcinoma SGC-7901 cells were exposed to alisol B 23-acetate at different concentrations. The inhibitive rate of cell proliferation was measured by MTT assay and the cell growth curve was set up，SGC-7901 cells were dyed by cation fluorescence carbocyanine JC-1，the mitochondrial membrane potential was determined by the inverted fluorescence microscope and flow cytometer，and the succinate dehydrogenase（SDH） activity was detected by microplate reader. Results Alisol B 23-acetate at 3.125 μg/mL promoted cell proliferation，while alisol B 23-acetate at 25-100 μg/mL inhibited the proliferation of SGC-7901 cell line in a dose-dependent manner. Compared with the normal control group（NCG），the changes of red fluorescence weakening（or green fluorescence enhancing） in alisol B 23-acetate groups were not obvious. The proportion of single positive cells was 3.5 %，3.6 %，3.7 % and 4.0 % in NCG，and low-dose，medium-dose，and high-dose ABA groups，respectively，and the differences of NCG with medium- and high-dose ABA groups were significant（P＜0.05，P＜0.01）. SDH activities of NCG，and low-dose，medium-dose，and high-dose ABA groups were 0.045，0.058，0.080，0.100 μmol•min-1，respectively，and the differences of NCG with medium- and high-dose ABA groups were significant（P＜0.05，P＜0.01）. Conclusion Low dose of alisol B 23-acetate would promote SGC-7901 cell proliferation，while medium dose and high dose of alisol B 23-acetate would inhibit SGC-7901cells proliferation and accelerate SGC-7901cells SDH activity. It is implied that alisol B 23-acetate could improve the mitochondrial energy metabolism，which lay the foundation for investigating the therapeutic mechanism of Rhizoma Alismae on improving mitochondrial energy metabolism.

R285.5

国家自然科学基金（81102875）；福建省省属公益类科研院所基本科研专项（2013R0034-3，2014R1031-7）；福建省卫生系统中青年骨干人才培养项目（2014-ZQN-JC-9）。