目的 研究淫羊藿苷对氧糖剥夺PC12细胞所致炎症损伤的保护作用。方法 采用PC12细胞复制氧糖剥夺2 h细胞损伤模型。实验分成6组，即正常组，模型组，尼莫地平组（10-5 mol•L-1），淫羊藿苷高、中、低剂量组（10-5，10-6，10-7 mol•L-1）。将淫羊藿苷各剂量组预给药1 h加氧糖剥夺期间给药2 h后，用MTT法检测细胞活力，ELISA法检测细胞上清液的肿瘤坏死因子-α（TNF-α）、白细胞介素-lβ（IL-1β）、白细胞介素-6（IL-6）含量。结果 淫羊藿苷各剂量组均能显著降低氧糖剥夺PC12细胞上清液的TNF-α、IL-1β、IL-6含量，提高细胞的活力，与模型组比较，差异均有显著性意义（P＜0.05，P＜0.01）。结论 淫羊藿苷能减轻氧糖剥夺PC12细胞所致的炎症损伤，具有细胞保护作用。

Objective To evaluate the protective effects of icariin on inflammatory injury of PC12 cells induced by oxygen glucose deprivation（OGD）. Methods The ischemic stroke model of PC12 cells was induced by OGD for 2 h. PC12 cells were divided into normal group，model group，Nimodipine（10 μmol•L-1） group，and high-，medium- and low-dosage icariin groups（10-5，10-6，10-7 mol/L icariin respectively）. Icariin groups were pretreated with the corresponding concentrations of icariin for one hour and then were treated with icariin for 2 hours during OGD. After the treatment，cell viability was measured using MTT assay. Tumor necrosis factor alpha（TNF-α），interleukin -l beta（IL-1β） and interleukin -6（IL-6） contents in PC12 cell supernatant were analyzed using enzyme-linked immunosorbent assay（ELISA）. Results Icariin at the concentrations of 10-5，10-6，10-7 mol/L significantly decreased TNF-α，IL-1β，IL-6 contents in cell supernatant and increased the cell viability of PC12 cells following OGD injury（vs model group，P＜0.05，P＜0.01）. Conclusion Icariin could reduce inflammatory damage of PC12 cells induced by OGD，and plays a protective role in the injured PC12 cells.

R285.5

教育部新世纪优秀人才支持计划（NCET-11-0927）；贵州省联合基金重点项目（黔科合J字LKZ［2013］04号）；遵义医学院博士启动基金（F-590）。