目的 采用基于核磁共振的代谢组学方法研究高果糖喂养对白脂肪代谢组的影响。方法 选取16只Wistar大鼠，将其适应1周后随机分为正常对照组和模型组，每组8只。采用100 g•L-1果糖水饲喂8周建立胰岛素抵抗（insulin resistance，IR）模型，对照组给予与模型组等体积的纯净水。8周末取大鼠白脂肪组织，运用主成分方法（principal component analysis，PCA）对白脂肪组织水溶性提取液的核磁共振氢谱（1H-NMR）谱进行分析。结果 与正常对照组比较，模型组大鼠的白脂肪组织代谢组明显变化，其中丙氨酸、氧化三甲胺/甜菜碱、胆碱和丙三醇含量升高，乳酸、脂质、谷氨酸、甘油磷脂胆碱/磷脂胆碱和不饱和脂类含量降低，差异均有统计学意义（P＜0.05，P＜0.01）。结论 采用核磁共振代谢组学方法研究高果糖对白脂肪代谢组的影响，阐明了IR状态下白脂肪组织提取液的代谢特征及其相应生化代谢过程的改变。

Objective To study the metabolomics changes of white adipose tissues in insulin resistance rats induced by fructose feeding by using 1H nuclear magnetic resonance（1H-NMR）. Methods Sixteen Wistar rats were evenly divided into control group and model group after adaptive feeding for one week. Rats in the model group were given 100 g/L of fructose water for 8 weeks to induce insulin resistance，and rats in the control group were given pure water in the same volume as the model group. At the end of the 8th week，the white adipose tissues were isolated and the 1H-NMR spectrum of each sample was collected for principal component analysis. Results Compared with the control group，the metabolomics of the white adipose tissues in model rats had obvious changes，showing as higher levels of alanine，trimethylamine oxide/betaine，choline and glycerol，and lower levels of lactic acid，lipid，glutamate，choline glycerophosphate/phosphatidylcholine，and unsaturated lipid，and the differences were statistically significant. Conclusion 1H-NMR based on metabonomics method can show the metabolic characteristics of white adipose tissues in insulin resistance rats induced by high fructose feeding，and provide biochemical changes of white adipose tissues.

R285.5

国家自然科学基金（21005022）。