目的 研究补骨脂素对A375细胞黑素合成的影响以及丝裂原活化蛋白激酶（MAPK）信号通路的作用。方法 将补骨脂素作用于A375细胞，采用MTT法检测细胞活性；NaOH裂解法检测黑素量；多巴氧化法检测酪氨酸酶（TYR）活性；RT-PCR法测定TYR、酪氨酸酶相关蛋白-1和酪氨酸酶相关蛋白-2（TRP-1、TRP-2）以及ERK1、ERK2、JNK2这3个MAPK信号通路关键蛋白激酶的mRNA表达。结果 补骨脂素在安全剂量下可显著抑制A375细胞黑素合成及TYR活性，1 μmol·L-1补骨脂素能明显下调A375细胞TYR、TRP-1、TRP-2以及ERK1、ERK2、JNK2 mRNA表达。结论 推测补骨脂素通过抑制ERK1、ERK2、JNK2的表达来抑制TYR活性和/或下调TYR、TRP-1及TRP-2 mRNA表达，抑制A375细胞的黑素合成。

Objective To observe the effect of psoralen on A375 cells of melanogenesis and MAPK signal pathway. Methods MTT test was used to test the cell activity，NaOH schizolysis method was applied for the detection of melanin amount，and L-dopa oxidation method was used to detect the activity of tyrosinase（TYR）. RT-PCR was applied to measure the mRNA expression levels of TYR and tyrosinase related proteins TRP-1，TRP-2，and the mRNA expression levels of MAPK signal pathway protein kinase of ERK1，ERK2 and JNK2. Results Psoralen in safe dose could significantly suppress melanin formation and TYR activity in A375 cells. Psoralen（1 μmol·L-1） could significantly down-regulate mRNA expression levels of TYR，TRP-1，TRP-2，ERK1，ERK2 and JNK2. Conclusion Psoralen can significantly suppress melanogenesis of A375 cells，and the mechanism is probably associated with the inhibition of the expression of ERK1，ERK2 and JNK2，and with the down-regulation of TYR，TRP-1 and TRP-2 mRNA.

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国家自然科学基金面上项目（81274035）；黑龙江省自然科学基金面上项目（D201234）；黑龙江省博士后科研启动金（LBH-Q13162）；黑龙江中医药大学优秀创新人才支持计划资助。