目的 探讨苍术苷（ATR）抑制氧化磷酸化电子传递链可能的作用靶点。方法 将制备好的线粒体悬液分为对照组、N -乙酰- L -半胱氨酸（NAC）组、鱼藤酮（ROT）组、NAC和ROT联合组，每组再分为4小组，分别用0，20，40，100 μmol·L-1的ATR处理。测定各组线粒体呼吸链中4种蛋白质复合体和ATP酶的活性。结果 ATR能够抑制复合体Ⅰ、Ⅳ和2种ATP酶的活性，且其抑制作用和剂量相关。结论 ATR可能影响呼吸链电子传递的始端复合体Ⅰ、末端状态复合体Ⅳ和ATP酶活性，最终抑制ATP的形成。

Objective To research the possible targets of atractyloside for inhibiting respiratory chain of oxidative phosphorylation. Methods The mitochondria suspension was divided into 4 groups，namely blank control group，N-acetyl-L-cysteine（NAC） group，rotenone（ROT） group，and NAC-ROT combination group. And then each group was divided into 4 subgroups，which were treated with 0，20，40，100 μmol·L-1 of atractyloside respectively. The activities of adenosine triphosphate（ATP） enzymes and 4 complexes at respiratory chain of each group were tested. Results Atractyloside could inhibit the activities of complexⅠand Ⅳ at respiratory chain，and Na+-K+-ATPase and Ca2+-Mg2+-ATPase. The effects were dose-dependent. Conclusion It suggested that atractyloside can inhibit the activities of complexⅠat initial point and ATP enzymes and complex Ⅳ at end point of respiratory chain，which results into the inhibition of ATP production.

R285.5

中国科学院重点部署项目“中药（民族药）标准化研究”（KSZD-EW-Z-004）；国家重点基础研究发展计划项目“973计划”项目（2009CB522804）。