目的 建立同时测定金归洗液中绿原酸和蛇床子素含量的高效液相色谱（HPLC）法。方法 采用HPLC法，色谱柱为Agilent ZORBAX SB-C18（4.6 mm×250 mm，5 μm）；流动相为乙腈-0.2 %磷酸水溶液（梯度洗脱），流速为1.0 mL·min-1；检测波长为324 nm；柱温为30 ℃。结果 金归洗液中绿原酸和蛇床子素分别在15.23~152.27 μg·mL-1（r=0.9999），4.35~43.52 μg·mL-1（r=0.9999）范围内浓度与峰面积呈良好线性关系，其平均加样回收率分别为98.13 %，RSD=1.51 %；98.69 %，RSD=0.92 %（n=6）。结论 该方法简单、准确、重复性好，可用于金归洗液中绿原酸和蛇床子素含量的同时测定。

Objective To establish a HPLC method for the simultaneous determination of chlorogenic acid and osthole in Jingui Lotion. Methods The HPLC method was performed on a ZORBAX SB - C18（4.6 mm×250 mm，5 μm）column. The mobile phase was a solution of acetonitrile -0.2 % phosphoric acid solution（gradient elution） at a flow rate of 1.0 mL·min-1. The detection wavelength was 324 nm，and the column temperature was 30 ℃. Results A good linearity of chlorogenic acid was shown in the range of 152.27 ~15.23 μg·mL-1（r=0.9999），and that of osthole was in the range of 4.35~43.52 μg·mL-1（r=0.9999）. The average recovery rate was 98.13 % with RSD 1.51 % for chlorogenic acid，and was 98.69 % with RSD 0.92 %（n=6） for osthole. Conclusion The established method is simple，accurate and with good reproducibility，which can be used for the simultaneous determination of osthole and chlorogenic acid in Jingui Lotion.

R284.1