目的 从脑-肠互动的角度，探讨百合总皂苷、知母总皂苷、百合知母总皂苷治疗肠易激综合征（irritable bowel syndrome，IBS）的疗效及其作用机制。方法 采用慢性束缚应激刺激配合夹尾刺激制备IBS模型，将60只成年雄性SD大鼠，随机分为6组，即正常组、IBS模型组、得舒特组（15 mg·kg-1）、百合总皂苷组（100 mg·kg-1）、知母总皂苷组（220 mg·kg-1）及百合知母总皂苷组（240 mg·kg-1），每组10只。观察大鼠在2，4，6周时的排便粒数和腹部撤回反射（AWR）评分。采用HE染色、醛品红-橙黄G特殊染色，检测结肠组织病理学变化及肥大细胞数量，酶联免疫吸附法（ELISA）分别检测大鼠血清降钙素基因相关肽（CGRP）、血管活性肠肽（VIP）含量的变化。结果 IBS模型组大鼠排便量增加，内脏敏感性增高，与正常组比较，差异有显著性意义（P＜0.01）；与IBS模型组比较，百合总皂苷组、知母总皂苷组、百合知母总皂苷组、得舒特组对IBS大鼠均有明显的治疗作用，使IBS模型大鼠结直肠部位肥大细胞数量以及血清CGRP、VIP的含量明显降低（P＜0.05，P＜0.01）。结论 百合总皂苷、知母总皂苷、百合知母总皂苷能减少IBS模型大鼠结直肠部位肥大细胞数量，降低血清CGRP、VIP的含量，从而对IBS起到一定的治疗作用。

Objective To investigate the therapeutic effect and mechanism of saponins of Bulbus Lilii，Rhizoma Anemarrhenae and their combination for irritable bowel syndrome（IBS） based on the interaction between brain and intestine. Methods IBS rat model was induced by chronic restraint stress and tail-clamping stress. Sixty male adult SD rats were randomly divided into normal control group，IBS model group，positive drug group（Dicetel at the dose of 15 mg·kg-1），Lilium saponins group（100 mg·kg-1），Timosaponin group（220 mg·kg-1） and Lilium saponins-Timosaponin group（240 mg·kg-1），10 rats in each group. On experimental week 2，4，6，the number of defecation grains and abdominal withdrawal reflex（AWR） scores of the rats were observed，HE staining and aldehyde fuchsin-orange G special staining were used for detecting colorectal histopathological changes and the number of mast cells，and enzyme-linked immunosorbent assay（ELISA） were used to detect serum calcitonin gene-related peptide（CGRP） and vasoactive intestinal peptide（VIP） contents. Results In the model group，the number of defecation grains and visceral sensitivity of rats increased，and the difference was statistically significant as compared with the normal group（P＜ 0.01）. Compared with IBS model group，Lilium saponins group，Timosaponin group，Lilium saponins-Timosaponin group and Dicetel group had obvious therapeutic effect for IBS，and decreased the number ofcolorectal mast cells and rectum and the serum contents of CGRP and VIP in rats（P＜ 0.05 or P＜ 0.01）. Conclusion Lilium saponins，Timosaponin，Lilium saponins-Timosaponin can reduce the number of colorectalmast cells and the serum contents of CGRP and VIP，thereby exerting therapeutic effect for IBS.

R285.5

国家自然科学基金资助项目（81273897）。