目的 研究芍苓汤对心得安豚鼠模型的影响及对脂多糖（LPS）诱导人永久分化表皮角质细胞（HaCaT细胞）产生炎症因子IL-6、IL-17A和IL-22 mRNA表达的调控作用。方法 以Baker评分法观测芍苓汤对豚鼠心得安模型的作用，实时荧光定量PCR（RT-Q-PCR）检测芍苓汤对LPS诱导HaCaT细胞IL-6、IL-17和IL-22 mRNA表达水平。结果 芍苓汤能够显著改善心得安诱发豚鼠的银屑病样病变，并显著下调LPS诱导的IL-6 mRNA表达。结论 芍苓汤具有治疗银屑病的药效，其作用机制与下调IL-6 mRNA表达水平有关。

Objective To observe the effect of Shaoling Decoction（SLD） on guinea pigs propranolol-induced psoriasis- like model and to investigate its regulatory effect on mRNA expression of IL-6，IL-17A and IL-22 in HaCaT keratinocyte cell line stimulated by ipopolyasccharide（LPS）. Methods Baker scoring method was used for the evaluation of Shaoling Decoction on the guinea pigs propranolol-induced psoriasis-like model，and real time fluorescence quantitative PCR（RT-Q-PCR） was applied for the detection of mRNA expression of IL-6，IL-17A and IL-22 in HaCaT line stimulated by LPS. Results Shaoling Decoction had an obvious effect on ameliorating the psoriasis-like pathological changes in guinea pigs，and on down-regulating IL-6 mRNA expression in LPS-activated HaCaT cell line. Conclusion Shaoling Decoction has certain therapeutic effect for psoriasis，and its mechanism may be related with down-regulation of IL-6 mRNA expression.

R285.5

广东省自然科学基金项目（10251040701000001）；广东省科技厅项目（2010A03010008，2011B032200009，2011A08030004）；广州市科技局项目（2010J-E031）。