目的 观察附子配伍不同比例大黄对附子毒性和药效的影响。方法 ICR小鼠，40 mL·kg-1体质量灌胃给药，平行比较附子和附子配伍大黄的急性毒性，测定其半数致死量（LD50）；SD大鼠10 mL·kg-1体质量灌胃给药，平行比较附子和附子配伍大黄的心脏毒性；采用高效液相色谱法测定附子与大黄配伍前后乌头类生物碱成分的含量；通过小鼠耳肿胀试验、醋酸扭体试验和回阳救逆试验考察附子配伍大黄对附子药效的影响。结果 随着大黄配伍比例的增高，附子急性毒性LD50有增加趋势，大黄对附子心脏心律失常抑制率逐渐增大，附子配伍大黄后主要乌头类生物碱成分含量降低，但附子配伍大黄后对附子抗炎、镇痛和回阳救逆药效无明显影响。结论 大黄对附子毒性具有剂量依赖性拮抗作用，大黄配伍附子在减轻附子毒性的同时不影响附子的药效，附子配伍大黄减毒时应该考虑大黄的配伍比例。

Objective To study the effects of Radix et Rhizome Rhei in different combination ratio on the toxicity and efficacy of Radix Aconiti. Methods Acute toxicity was evaluated by detecting the median lethal dose（LD50） in ICR mice treated with gastric infusion of the decoction of Radix et Rhizome Rhei combined with Radix Aconiti in the dose of 40 mL·kg-1 body weight. Heart toxicity was evaluated in SD rats after treat with gastric infusion of the decoction of Radix et Rhizome Rhei combined with Radix Aconiti in the dose of 10 mL·kg-1 body weight. HPLC was applied for the determination of aconitine in Radix et Rhizome Rhei and Radix Aconiti before and after compatibility. The pharmacodynamic research of Radix et Rhizome Rhei combined with Radix Aconiti was also carried out through mice auricular swelling test，acetic acid body writhing test，and test of reviving yang for resuscitation. Results With the increase of the combined use of Radix et Rhizome Rhei，both LD50 value in acute toxicity test and arrhythmia inhibition rate of Radix Aconiti were positively increased，while the contents of the toxic components in the water-extract of their combination were lower than those in the water-extract of Radix Aconiti alone. The results of pharmacodynamic research showed that the combined use of Radix et Rhizome Rhei had no effect on the anti-inflammatory action，analgesic action and actions of reviving yang for resuscitation of Radix Aconiti. Conclusion Radix et Rhizome Rhei appeared to have dose-dependent antagonistic action on the toxicity of Radix Aconiti，but has on effect on the pharmacodynamic action of Radix Aconiti. When used for decreasing the toxicity of Radix Aconiti，the dose of the combined use of Radix et Rhizome Rhei should be taken into account. dose of rhubarb should be considered when it is combined with Radix aconiti carmichaeli for detoxification.

R285.5

国家重点基础研究发展计划（973）计划项目（2009CB522800）。