目的 观察补肾清毒方对HBV转基因小鼠外周血乙肝病毒DNA（HBV-DNA）、干扰素-γ（IFN-γ）及白介素-17A（IL-17A）的影响。方法 将50只HBV转基因小鼠随机分为模型组，补肾清毒方高、中、低剂量组，拉米夫定组，另取10只非HBV转基因小鼠为正常对照组。采用荧光定量PCR方法检测药物干预前后小鼠外周血HBV-DNA水平；酶联免疫吸附试验（ELISA）检测药物干预前后小鼠血清IL-17A及IFN-γ的水平。结果 与正常对照组比较，模型组IL-17A显著升高（P＜ 0.01），IFN-γ也明显升高（P＜ 0.05）。与模型组比较，各药物干预组小鼠血清IL-17A水平不同程度降低（P＜ 0.01），其中以补肾清毒方高剂量组和中剂量组降幅最为明显（P＜ 0.01），并且随用药时间的延长IL-17A水平进一步降低；各药物干预组血清IFN-γ水平升高（P＜ 0.05），其中以补肾清毒方高、中剂量组升高最为显著（P＜ 0.01），且随用药时间延长有所提高。与模型组比较，各药物干预组小鼠外周血HBV-DNA变化不大（P ＞ 0.05）。结论 补肾清毒方对乙肝有一定的治疗作用，其作用机理可能与升高IFN-γ水平、降低IL-17A表达有关。

Objective To observe the effect of Bushen Qingdu decoction（BQD） on peripheral blood hepatitis B virus DNA（HBV-DNA） and serum interferon gamma（IFN-γ） and interleukin 17A（IL-17A） of HBV transgenic mice. Methods Fifty HBV transgenic mice were randomly divided into normal control group，model group，lamivudine group，and high-，middle-，and low-dosage BQD groups. Before and after drug intervention，the level of HBV-DNA was detected by fluorescence quantitative PCR，and serum IFN-γ and IL-17A levels were detected by enzyme linked immunosorbent assay（ELISA）. Results Compared with the normal control group ，the levels of IL-17A and IFN-γ were significantly increased（P＜ 0.01 or P＜ 0.05） in the model group. IL-17A was decreased and IFN-γ was increased to various degrees in the medication groups（P＜ 0.01 compared with the model group） in time-dependent manner， and the decrease of IL-17A in high-dosage BQD group and the increase of IFN-γ in high- and middle-dosage BQD groups were more obvious（P＜ 0.01，P＜ 0.05）. There was no significant changes in the expression of HBV-DNA between medication groups and model group. Conclusion BQD is effective for the treatment of chronic hepatitis B，and its mechanism is probably related with the increase of IFN-γ and with the decrease of IL-17A expression.

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国家自然科学基金面上项目（81173255）；广州中医药大学中医临床基础学科国家特色重点学科项目。