目的 初步研究葛根素与牛磺酸配伍对红细胞膜稳定性、膜流动性及形态影响。方法 采用体外溶血实验，比较葛根素与牛磺酸配伍前后红细胞溶血的程度；采用荧光染料技术，比较葛根素与牛磺酸配伍前后红细胞膜流动性的变化；体内试验以血细胞涂片观察葛根素与牛磺酸配伍前后对红细胞形态的影响。结果 葛根素能直接导致红细胞溶血，牛磺酸可抑制葛根素所致的溶血现象。葛根素能显著提高红细胞膜流动性，牛磺酸对葛根素此作用无明显影响。静脉注射葛根素可引起部分红细胞呈棘形，牛磺酸能抑制葛根素对红细胞形态的改变。结论 牛磺酸体外能对抗葛根素的溶血现象，体内可抑制葛根素诱导的红细胞形态异常。

Objective To measure the effect of the combination of puerarin and taurine on membrane stability，membrane fluidity and membrane histology of erythrocytes. Methods Hemolysis tests in vitro were performed for puerarin and puerarin-taurine mixture. Membrane microviscosity of erythrocytes was tested after treating with puerarin or puerarin-taurin mixture. Erythrocytes smear of the rats treated with intravenous injection of puerarin or puerarin-taurine mixture was used for the observation of morphological changes of erythrocytes under light microscope. Results Puerarin caused obvious hemolysis directly while taurin had no effect on the hemolysis induced by puerarin. Both puerarin and puerarin-taurin mixture showed similar effect on the membrane microviscosity of erythrocytes. Intravenous injection of puerarin induced thorn-like morphological changes of some erythrocytes in rats，while taurine showed an inhibition of morphological changes of some erythrocytes. Conclusion Taurine could suppress the hemolysis induced by puerarin in vitro，and relieve the morphological changes of erythrocytes induced by puerarin.

R285.5

教育部高等学校博士学科点专项科研基金联合资助课题（20114425120012）；广东省自然科学基金博士启动基金项目（S2011040003141）。