目的 建立同时测定杏香兔耳风制剂中8种咖啡酰奎宁酸有效成分含量测定的方法，并比较不同厂家生产的杏香兔耳风单方与复方制剂的含量差 异。方法 采用Cosmosil RP-C18色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-0.1%甲酸水溶液，梯度洗脱；流速：1 mL·min-1；检测波长：328 nm；柱温：35 ℃； 结果 8 种被测咖啡酰奎宁酸成分分离度良好；各成分质量浓度与峰面积在测定范围内均呈良好的线性关系（r ＞ 0.9995）；重复性良好；平均加样回收率在98.14 %～ 101.73 %（RSD为1.26 %～2.73 %）（n=9）。所收集的不同厂家杏香兔耳风制剂中，酚酸含量相差较大；杏香兔耳风胶囊（厂家1）中咖啡酰奎宁酸的含量相对较高，其他厂 家的杏香兔耳风制剂酚酸含量相近。结论 该方法准确、简单，重复性良好，可用于不同杏香兔耳风制剂中8种酚酸的含量测定。

Objective To establish a method for the simultaneous determination of eight kinds of caffeoylquinic acids in different preparations of Ainsliaea frangrant Champ，and to compare the content difference in single herb and compound preparations of Ainsliaea frangrant Champ from different producers. Methods HPLC was performed on Cosmosil RP - C18 column（250 mm×4.6 mm，5 μm）with gradient elution，and acetonitrile-0.1 % formic acid aqueous solution was used as the mobile phase. Flow rate was 1 mL·min-1 and detection wavelength was set at 328 nm. The column temperature was 35 ℃. Results Good separation was shown in eight kinds of caffeoylquinic acids and good linear relationship was presented between concentration and peak area（r \> 0.9995）. The average recovery was from 98.14 %～101.73 %，and RSD was 1.26 %～2.73 %（n=9）. Great difference of the content of caffeoylquinic acids was shown in Ainsliaea frangrant Champ preparations：the capsule from producer 1 had higher contents of caffeoylquinic acids，but the contents of caffeoylquinic acids from the others were similar. Conclusion The established method is accurate，simple，and with good repeatability， which can be used for the simultaneous determination of 8 kinds of caffeoylquinic acids in different Ainsliaea frangrant Champ preparations.

R284.1

国家自然青年科学基金项目（81102787）；江西省青年科学基金计划重点项目（20133ACB21005）；江西中医药大学博士启动基金 （Y055），江西省卫生厅中 医药科研计划 （2012A026，2012A158）；江西中医药大学科研基金 （ZX1001）。