目的 研究不同产地种源铁皮石斛高效液相色谱（HPLC）特征图谱，并初步比较丹霞地貌种源（广东、福建、浙江、江西）、浙江本地种、铁 皮兰种（广西、云南）特征图谱的差异。方法 采用Zorbax SB Aq色谱柱；流动相为乙腈-体积分数为0.2 %的甲酸溶液（梯度洗脱）；检测波长为270 nm；柱温为35 ℃；流 速为1.0 mL·min-1。采用国家药典委员会中药色谱指纹图谱相似度评价系统软件（2004A版）生成对照图谱。结果 10批丹霞种源样品标示出37个特征共有峰，以对照图谱为 对照，10批样品的相似度为0.907~0.972；10批丹霞种源生成的对照图谱与5批浙江本地种对照图谱、5批铁皮兰种对照图谱的相似度分别为0.855，0.700。结论 分析方法准 确可靠，重复性好；提出了铁皮石斛“丹霞铁皮种”、“浙江本地种”、“铁皮兰种”的种源概念；3种铁皮石斛种源主要成分群基本一致，显示铁皮石斛内在成分的稳定性 ；不同道地产地种源成分群比例以及少数特征峰存在差异，又为区分丹霞种源、浙江本地种、铁皮兰种区域特征提供一定依据。

Objective To study and compare the HPLC characteristic spectrum of Dendrobium officinale from provenance sources of Danxia landform（Guangdong，Fujian，Zhejiang and Jiangxi），Tiepilan（Guangxi，Yunnan）and Zhejiang native．Methods HPLC was performed on Zorbax SB Aq column ，the acetonitrile-methanoic acid solution（φ=0.2 %）at gradient elution was employed as a mobile phase，detection wavelength was 270 nm，column temperature was 35 ℃，and flow rate was 1.0 mL·min-1. The contrast spectrum was established by Chinese herbal medicine chromatographic fingerprint similarity evaluation software issued by Chinese pharmacopeia committee. Results Thirty-seven common peaks were separated from Danxia landform species of Dendrobium officinale，and the similarities of 10 batches were 0.907~0.972. The similarities of common pattern of 10 batches of Danxia landform species with 5 batches of Tiepilan species and with 5 batches of Zhejiang native species were 0.700，0.855，respectively. Conclusion The established method is accurate and reliable，and has better reproducibility. The results put forward to the classification of Danxia landform species，Tiepilan species and Zhejiang Native species. The three species have the similar main components，which show the stability of internal quality of Dendrobium officinale. However，variabilites exist in component peak areas ratio and some characteristic spectrum，which provide reference to distinguish the characteristics of Danxia landform species，Tiepilan species and Zhejiang Native species of Dendrobium officinale.

R284.1