目的 观察黄芩茎叶总黄酮（SSTF）对高浓度葡萄糖（HG）刺激的血管平滑肌细胞（VSMCs）增殖的抑制作用及可能的机制。方法 体外培养大 鼠胸主动脉VSMCs，HG诱导其增殖，观察SSTF对细胞增殖及迁移、细胞周期、醛糖还原酶（AR）mRNA及AR蛋白表达的影响。结果 与模型组比较，SSTF明显抑制HG诱导的VSMCs 增殖及迁移，抑制细胞周期进程（P＜ 0.05，P＜ 0.01），并呈时间和剂量依赖趋势；SSTF显著抑制细胞AR mRNA及AR蛋白表达（P＜ 0.05，P＜ 0.01）。结论 SSTF对HG刺 激的VSMCs增殖具有抑制作用，可能与降低AR表达有关。

Objective To investigate the inhibitive effect and possible mechanism of Scutellaria baicalensis stem-leaf total flavonoids （SSTF） on the proliferation of vascular smooth muscle cells（VSMCs） stimulated by high-concentration glucose（HG）. Methods Rat aorta pectoralis VSMCs were subcultured in vitro and were treated by HG to induce proliferation. And then we observed the effects of SSTF on the proliferation， migration and cell mitotic cycle of VSMCs as well as aldose reductase（AR） mRNA and protein expression in VSMCs. Results SSTF（400 mg·L-1）group had an obvious effect on inhibiting the proliferation and migration of VSMCs and the progress of cell cycle，and the effect was time- and concentration- dependent（P＜ 0.01）. The mRNA and protein expression levels of AR were also inhibited in SSTF（400 mg·L-1） group. Conclusion SSTF have inhibitive effect on VSMCs proliferation induced by HG through blocking the cell cycle progress，and the possible mechanism is related with the inhibition of AR expression.

R285.5