目的 观察自热式痹痛巴布剂对小鼠疼痛扭体潜伏时间、扭体次数，二甲苯所致小鼠耳廓肿胀及血液中5 - 羟色胺（5-HT）含量的影响。方法 取NIH小鼠50只，雌雄各半，随机分为5组：正常对照组（等量基质巴布剂）、扶他林乳胶组（0.78 g·kg-1）、活血止痛膏组（36.4cm2·kg-1）、痹痛巴布剂组（1.30 g· kg-1）和自热式痹痛巴布剂组（1.30 g·kg-1），观察给药后对小鼠腹腔注射乙酸刺激所致小鼠疼痛扭体的影响、对二甲苯所致小鼠耳廓肿胀的影响，并采用酶联免疫法测 定小鼠血清内5-HT含量的改变。结果 自热式痹痛巴布剂组与痹痛巴布剂组比较，小鼠疼痛扭体的次数减少，差异有显著性意义（P ＜ 0.01）。自热式痹痛巴布剂组与痹 痛巴布剂组比较，小鼠耳廓肿胀率降低，但无统计学意义（P ＞ 0.05）。自热式痹痛巴布剂组5-HT与痹痛巴布剂组比较含量升高（P ＜ 0.05）。结论 适当的温热治疗能使 小鼠疼痛扭体次数减少以及二甲苯所致小鼠耳廓肿胀率下降，体内血清5-HT水平增高。

Objective To observe the effect of self-heating Bitong cataplasm on pain-induced mice writhing latency and frequency， xylene-induced mice auricular swelling and mice blood 5-hydroxytryptamine（5-HT）level. Methods Fifty NIH mice，half being male，were evenly randomized into 5 groups，namely voltaren emulsion group（external application of voltaren 0.78 g·kg-1，tid），Huoxue Zhitong ointment group（36.4 cm2·kg-1，qd ），Bitong cataplasm group（one piece per day，1.30 g·kg-1），self-heating Bitong cataplasm group（one piece per day，1.30 g·kg-1），and normal control group（external application of same volume of cataplasm）. The external application lasted for 7 days. The effect of the medication on mice pain writhing induced by acetic acid and xylene-induced mice auricular swelling was observed. The changes of mice blood 5-HT level were observed by enzyme-linked immunosorbent assay. Results Pain writhing frequency was decreased（P＜0.01）and blood 5-HT level was increased（P＜0.05）in self- heating Bitong cataplasm group as compared with those in Bitong cataplasm group. However，the difference of incidence of auricular swelling was insignificant between self-heating Bitong cataplasm group and Bitong cataplasm group（P \> 0.05）. Conclusion The proper warming treatment can reduce mice pain-induced writhing frequency and xylence-induced mice auricular swelling，and increase blood 5-HT level.

R285.5

广东省科技计划项目（2012B031800414）。