目的 观察健脾活血袪湿方对肝硬化腹水大鼠肝功能及肝组织水通道蛋白9（AQP 9）表达的影响。方法 将Wistar大鼠，随机分为6组，即正常 组，模型组，健脾活血祛湿方高、中、低剂量组（52，26，16.25 g·kg-1），秋水仙碱组（100 μg·kg-1），观察大鼠的一般情况，测定腹水量、检测谷丙转氨酶（ALT） 、谷草转氨酶（AST）、白蛋白（ALB）的含量及AQP 9 mRNA转录与蛋白翻译水平。结果 健脾活血祛湿方对肝硬化腹水大鼠症状有明显的改善作用，能促进模型大鼠腹水消退 、改善相应肝功能指标，具有良好的保肝作用；与模型组比较，健脾活血祛湿方组AQP 9 mRNA转录与蛋白翻译水平均有不同程度的升高，作用强度与用药剂量呈正相关。结 论 健脾活血祛湿方良好的保肝作用及对AQP 9的升高作用是其治疗肝硬化腹水的靶点之一。

Objective To study the influence of Jianpi Huoxie Qusshi prescription（JHQP）on the hepatic function and aquaporin 9（AQP9） expression in the cirrhotic rats with ascites. Methods Healthy Wistar rats were evenly randomized into blank control group，model group，colchine（100 μg·kg-1）group，and high-，middle- and low-dose JHQP groups（in the dosage of 52，26，16.25 g·kg-1，respectively）. The rats were given oral administration of the corresponding medicine once a day for 4 continuous weeks. After treatment，the general health status of the rats were observed， the amount of ascites was tested，and the serum levels of ALT，AST and ALB as well as AQP9 mRNA and protein expression were detected. Results JHQP could obviously improve the general health state of the rats，promote the subsidence of ascites and improve the indexes of hepatic function，indicating that JHQP had satisfactory protective effect on liver. The expression levels of AQP9 mRNA and protein were increased to various degrees in JHQP groups as compared with those in the model group，and the increase was positively correlated with the dosage. Conclusion JHQP shows good hepatic protection for cirrhotic rats with ascites，and the therapeutic mechanism may be related with the increase of AQP9 expression.

R285.5

湖南省中医药管理局中医药科学研究项目（2013116）。