目的 探讨敛肝熄风养血濡筋方对帕金森病（Parkinson’s disease，PD）大鼠黑质抗凋亡蛋白-2（Bcl-2）、促凋亡蛋白（Bax）表达的影响 。方法 采用6-羟基多巴胺（6-OHDA）纹状体左侧两点注射法复制PD大鼠模型，术后将大鼠随机分为空白对照组、模型组、美多巴组（0.075 g·kg-1）、敛肝熄风养血濡筋 方（LXYR）（32，16，8 g·kg-1）剂量组，连续灌胃4周。实验结束后，免疫组化法检测各组大鼠黑质组织Bcl-2 和Bax蛋白的表达。结果 与空白对照组比较，模型组Bcl-2 和Bax蛋白表达增加（均P＜ 0.01）。与模型组比较，敛肝熄风养血濡筋方高、中剂量组均能增加Bcl-2蛋白表达，降低Bax 蛋白表达，差异有统计学意义（P＜ 0.05），其 中以高剂量组改变最为显著（P＜ 0.01），而低剂量组及美多巴组与模型组比较差异无统计学意义（P ＞ 0.05）。结论 敛肝熄风养血濡筋方可通过调节Bcl-2/ Bax表达数 量而起到保护黑质多巴胺能神经元的作用。

Objective To observe the effects of Liangan Xifeng Yangxue Rujin（LXYR）Formula，a Chinese herbal formula with the actions of astringing liver to extinguish wind and nourishing blood to moisten the tendon，on Bcl-2 and Bax protein expression in substantia nigra of rats with Parkinson’s disease（PD）. Methods The hemilateral PD model was reproduced by stereotaxically two-site injection of 6-hydroxydopamine（6-OHDA）into the left side of rat striatum. Rats were randomized into five groups，namely normal control group，model group，madopar group，LXYR groups（32，16，8 g ·kg-1）. The oral administration of the intervention medicine lasted for 4 weeks. At the end of the experiment，Bcl-2 and Bax protein expression levels were detected by immunohistochemistry. Results Compared with the normal control group，cerebral Bcl-2 and Bax expression was increased in the model group（P＜ 0.01）. In contrast with the model group，high-and middle-dose LXYR decreased Bax expression and increased Bcl-2 expression（P＜ 0.05 ，P＜ 0.01）. However，the expression levels of Bax and Bcl-2 in low-dose LXYR group and madopar group did not differ from the model group（P＞0.05）. Conclusion LXYR Formula could protect nigrostriatal dopaminergic neurons from apoptosis through regulating Bcl-2 and Bax expression.

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“十一五”国家科技支撑计划重大项目（2006BAI04A11-1）。