目的 研究山慈菇-蜂房药对对人乳腺癌MDA-MB-231细胞侵袭能力的影响及其作用机制。方法 噻唑蓝（MTT）法检测山慈菇-蜂房药对大鼠含药 血清对细胞活力的影响，Transwell小室法测定其侵袭力，实时荧光定量PCR（RT-PCR）法检测细胞基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶组织抑制剂-1（TIMP-1）基 因的表达。结果 山慈菇-蜂房药对组MDA-MB-231细胞体外生长杀伤率为35.86 %；能明显抑制MDA-MB-231 细胞的侵袭能力（P＜ 0.01），其抑制率为34.8 %；MMP-9 mRNA明 显降低，TIMP-1 mRNA明显升高，MMP-9 mRNA/ TIMP-1 mRNA值显著降低（P＜ 0.05，P＜ 0.01）。结论 山慈菇-蜂房药对能够抑制MDA-MB-231 细胞侵袭能力，其机制可能与 下调MMP-9mRNA表达，上调TIMP-1mRNA的表达，降低MMP-9 mRNA/ TIMP-1 mRNA比值有关。

Objective To explore the inhibitive effects and mechanism of Chinese herbal pair of Pseudobulbus Cremastrae seu Pleiones （Shancigu） and Nidus Polistis Mandarini（Lufengfang） on in-vitro invasion and metastasis of human breast cancer MDA-MB-231 cells. Methods The effect of serum containing Shancigu-Lufengfang on MDA-MB-231 cell viability was determined by methyl thiazolyl tetrazolium（MTT） assay. The invasion ability of the cells was measured by Transwell chamber. Real-time fluorescence polymerase chain reaction（RT-PCR） technique was used to evaluate the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinases-1 （TIMP-1） in MDA-MB-231 cells. Results Chinese herbal pair of Shancigu-Lufengfang showed an in-vitro killing rate of 35.86 % on MDA-MB-231 cells，and could obviously inhibit the invasion ability of MDA- MB-231 cells（P＜ 0.01），the inhibition ratio being 34.8 %. MMP-9 mRNA expression was significantly decreased（P＜ 0.05），while TIMP-1 mRNA expression was obviously increased（P＜ 0.05），therefore the ratio of MMP-9 mRNA/TIMP-1 mRNA was reduced dramatically （P＜ 0.05，P＜ 0.01）. Conclusion Chinese herbal pair of Shancigu-Lufengfang could inhibit the invasion ability of MDA-MB-231 cells，and the inhibitive mechanism may be correlated with the down-regulation of MMP-9 mRNA and the UP-regulation of TIMP-1 mRNA，thus decreasing the ratio of MMP-9mRNA/ TIMP-1 mRNA.

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河南省科技厅2010年基础与前沿项目（102300410040）；金水区2011技术研究与开发经费支持项目（20113149）；河南中医学院2010年苗圃工程。