目的 筛选糖肾宁颗粒的提取工艺路线并优选水提部分工艺。方法 以KK-Ay小鼠制备糖尿病肾病模型，采用酶联免疫法测定24 h尿微量白蛋白含量，通过比较不同提取物给药前后微量白蛋白含量变化确定提取工艺路线；水提部分以加水倍数、提取时间、提取次数为主要影响因素，大黄酸转移率为评价指标，采用正交试验设计优化提取工艺条件。结果 路线2降低尿微量白蛋白含量效果最好；优选的糖肾宁方水提部分最佳提取工艺为加10倍水提取3次，每次1.5 h。结论 药效学试验确定了适宜的提取路线，优选的水提工艺条件稳定可行。

Objective To screen the extraction routine and to optimize the water extraction process for Tangshenning granules. Methods The 24-hour microamount urinary albumin of KK-Ay mice diabetic nephropathy（DN） model was detected by enzyme-linked immunosorbent assay（ELISA） and was compared before and after administration of the extracts extracted by different technology routine. With the volume of water added，extraction time and extraction frequency as the main influencing factor，and with the rhein extraction rate as the evaluation index，the water extraction process for Tangshenning granules was optimized. Results The No 2 extraction routine showed better effect on reducing microamount urinary albumin than the other two. The optimal water extraction process of No 2 extraction method was reflux with 10-fold water for 3 times，extraction for 1.5 h per time. Conclusion Pharmaceutical screening can be applied to establish the appropriate extract process of Tangshenning granules，and the optimized water extraction process is stable and feasible.

R284.1

北京市教育委员会产业转化项目（201325）；北京市教育委员会“首都中医药与护理学研究专项”课题（2012-2013，12ZYH09）。