目的 建立小柴胡颗粒中柴胡皂苷b2含量的测定方法。方法 采用HPLC法，phenomenex Luna色谱柱，以乙腈-0.2 %二乙胺（35∶65）为流动相，流速为1.0 mL·min-1，柱温30 ℃，检测波长254 nm。结果 柴胡皂苷b2线性范围 0.3632~3.6320 μg，R2=1.00；平均加样回收率为102.2 %，RSD为2.5 %。结论 该方法准确、稳定，重现性好，可用于小柴胡颗粒的质量控制。

Objective To establish a high performance liquid chromatography（HPLC）method for the determination of saikosaponin b2 in Xiaochaihu Granules. Methods HPLC was performed on Phenomenex Luna column. The mobile phase was a mixture of acetonitrile -0.2 % diethylamine（35∶65）. The detection wavelength was set at 254 nm. The flow rate was 1.0 mL·min-1 and column temperature was 30 ℃. Results The linear range of saikosaponin b2 was within 0.3632~3.6320 μg，R2=1.00. The average recovery was 102.2%，and RSD=2.5 %. Conclusion The established method is simple，stable and reproducible. It can be used for the quality control of Xiaochaihu Granules.

R284.1

国家自然科学基金项目（81102530）。