目的 研究生附子临床煎煮过程中双酯型乌头碱的溶出动态变化规律，建立双酯型乌头碱含量与煎煮剂量和煎煮时间的关系。方法 采用高效液相色谱法（HPLC），Technologies Athena C18-WP色谱柱（4.6 mm×250 mm，5 μm），流动相：甲醇（0.1 %三乙胺）-水（70∶30），流速：1.0 mL·min-1，柱温：35 ℃，检测波长：235 nm。结果 生附子水煎液中双酯型乌头碱水煎极不稳定，随煎煮时间的增加，乌头碱比例不断增加，4 h达到峰值。新乌头碱和次乌头碱相似，均有下降趋势，其中次乌头碱明显下降，0.5 h最高，4 h最低。结论 该方法学考察符合定量要求，结果准确，可说明生附子临床水煎液中双酯型乌头碱的溶出动态变化规律。

Objective To investigate the dynamic variation of diester diterpenoids alkaloids in raw Radix Aconite Lateralis during decocting in clinic，and to explore the relationship of diester diterpenoids alkaloids contents with decocting time and the dosage. Methods HPLC analysis was performed on a Technologies Athena C18-WP（4.6 mm×250 mm，5 μm） column with methanol（0.1 % triethylamine）-water（70∶30） as a mobile phase. The flow rate was 1.0 mL·min-1，column temperature was fixed at 35 ℃，and the wave length of detection was set at 235 nm. Results The diester diterpenoid alkaloids were extremely unstable during the decoction of raw Radix Aconite Lateralis，and the content of aconitine showed a tendency of increasing gradually，and then reached the peak value after decocting for 4 hours. The contents of mesaconitine and hypaconitine showed the same decreasing trend. Hypaconitine content arrived the highest after decocting for 0.5 h，and was the lowest after decocting for 4 h. Conclusion The established quantitative method is feasible and precise. It can illuminate the changes of diester diterpenoids alkaloids in Radix Aconite Lateralis during decocting in clinic.

R284.1

广东省科技计划重点项目（2010A03010003）；广东省中医药局科研课题项目（20121177）。