[关键词]
[摘要]
目的 建立巴戟天环烯醚萜苷类和蒽醌类成分HPLC指纹图谱。方法 采用ZOBAX SB Aq(250 mm×4.6 mm,5 μm)色谱柱;流动相为乙腈-0.1 %磷酸溶液(梯度洗脱);检测波长分别为235,280 nm;柱温为30 ℃;流速为1.0 mL·min-1。结果 标示出巴戟天环烯醚萜苷类和蒽醌类成分指纹图谱具有8个和7个共有峰。结论 市售巴戟天药材质量较稳定,本研究为巴戟天的质量控制提供了方法依据。
[Key word]
[Abstract]
Objective To establish HPLC fingerprint of iridoid and anthraquinone components of Radix Morindae Officinalis. Methods Zorbax SB Aq(250 mm×4.6 mm,5 μm) column was used with acetonitrile-0.1 % phosphoric acid solution as the mobile phase by gradient elution,the flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃. The detection wavelength was set at 235 nm and 280 nm respectively. Results Eight characteristic peaks of iridoid components and 7 characteristic peaks of anthraquinone components were separated from 11 batches of Radix Morindae Officinalis. Conclusion The quality of Radix Morindae Officinalis in the market is stable,and the results will provide a reference for the quality control of Radix Morindae Officinalis.
[中图分类号]
R284.1
[基金项目]