目的 探讨肿节风总黄酮对体外培养巨核细胞增殖的影响及其效应动力学。方法 肿节风总黄酮（0.39 g·kg-1）单次灌胃给予正常SD大鼠，给药后不同时间点采血制备含药血浆。采用兔抗大鼠血小板相关抗体建立大鼠骨髓巨核细胞增殖障碍模型，并将不同时间点的含药血浆作用于该模型，以巨核细胞数、细胞上清液中血小板生成素（TPO）、转化生长因子-β1（TGF-β1）含量为效应指标，绘制时间-效应动力学曲线，计算相关动力学参数。结果 肿节风总黄酮含药血浆可以显著提升巨核细胞数，肿节风总黄酮对巨核细胞数、上清液中TPO及TGF-β1的效应半衰期分别为：495.52，665.13和537.46 min，达峰时间分别为：60，90和40 min，平均驻留时间分别为：224.60，242.37和231.10 min，其中巨核细胞数与TPO含量的效应曲线之间有较好的相关性，时间上稍有滞后现象。结论 肿节风总黄酮含药血浆可显著促进大鼠骨髓巨核细胞的增殖，可能与培养体系中TPO的浓度密切相关。

Objective To investigate the effects of plasma containing total flavonoids from Herba Sarcandrae（TFHS） on the proliferation of rat bone marrow megakaryocytes（BMM） and to explore its pharmacodynamics. Methods SD rats were administrated TFHS at the dose of 0.39 g/kg for one time. At various time points after administration，the plasma containing TFHS was collected. BMM proliferation disorder model was established by using rabbit anti-rat platelet associated antibody，and then was treated with the plasma containing TFHS. With megakaryocyte count，serum thrombopoietin（TPO） and transforming growth factor beta 1（TGF-β1） as the effectiveness parameters，the pharmacodynamics of TFHS was studied. The apparent parameters of pharmacodynamics were estimated based on the time-effect curve. Results The plasma containing TFHS could significantly promote the number of megakaryocytes. The main pharmacodynamics parameters of the number of megakaryocytes，the levels of TPO and TGF-β1 were as follows：half life period t1/2 was 495.52，665.13 and 537.46 min，peak time Tmax was 60，90 and 40 min，mean residence time MRT（0-t） was 224.60，242.37 and 231.10 min，respectively. There was a satisfactory correlation between megakaryocyte count and TPO level，though mild retardation was shown in medication time. Conclusion The plasma containing TFHS could significantly promote the proliferation of rat bone marrow megakaryocytes，which may be associated with the regulation of TPO level in the culture supernatants.

R285.5

国家自然科学基金（81260653，81001662）；江西省自然科学基金（20114BAB215039）；江西省教育厅项目（GJJ13582）；江西省卫生厅项目（2013A006）。