目的 观察寿胎丸对去势大鼠血清雌激素水平和骨形态计量学参数的影响。方法 84只雌性SD大鼠随机分为6组，除假手术组外，其余5组建立去势模型（均行双侧卵巢切除术），寿胎丸高、中、低剂量组每天灌胃剂量分别为26.8，13.4，6.7 g·kg-1的药液，阳性对照药戊酸雌二醇组灌胃剂量为0.104 mg·kg-1的药液，假手术组与模型组予等量生理盐水灌胃。于术后12周处死动物，取静脉血检测血清雌二醇（E2）水平；获取右侧胫骨标本测定骨形态计量学参数。结果 与假手术组比较，模型组血清E2水平下降且差异具有统计学意义（P ＜0.05）；与模型组比较，寿胎丸高、中、低剂量组及戊酸雌二醇组血清E2水平均有明显升高（P ＜0.05）；寿胎丸高、中剂量组及戊酸雌二醇组血清E2水平与寿胎丸低剂量组比较差异均有统计学意义（P ＜0.05）。与假手术组及模型组比较，各给药组在骨矿化沉积率无明显变化（P \> 0.05）；与模型组比较，各给药组骨小梁面积百分数、骨小梁厚度、骨小梁密度、骨表面骨形成率、骨体积骨形成率升高，骨小梁分离度及破骨细胞数则降低，差异均有统计学意义（P ＜0.05）。血清E2水平与骨形态计量学中骨小梁面积百分数、骨小梁厚度、骨小梁密度骨小梁分离度、骨表面骨形成率、骨体积骨形成率、破骨细胞数明显相关（P ＜0.05）。结论 雌激素水平与绝经后骨质疏松骨组织的微观结构改变密切相关。寿胎丸中、高剂量可提高血清E2水平，改善骨组织微观结构、抑制骨结构退化、促进骨形成、抑制骨吸收。

Objective To observe the effects of Shoutai pills（SP） on estrogen level and bone histomorphometry parameters in ovariectomized rats. Methods A total of 84 female healthy SD rats were randomly divided into 6 groups. A sham operation group was set up，and rats in the other groups were given bilateral oophorectomy. SP at the dosages of 26.8，13.4，6.7 g·kg-1 and Progynova at the dosage of 0.104 mg·kg-1 were administered to the rats separately by intragastric administration according to the experimental design. Sham operation group and ovariectomized model group were given intragastric administration of equal volume of saline. The rats were executed 12 weeks after the operation. The estradiol level in venous blood was detected，and the bone histomorphometry parameters of right tibia were also evaluated. Results There was significant difference of estrogen level（P ＜0.05） between sham operation group and model group. Compared to the model group，estrogen level in all of the medicinal intervention groups were significantly increased（P ＜0.05）. Differences of estrogen level between high-，middle-dosage SP groups and Progynova group showed statistical significance（P ＜ 0.05）. Estrogen level in low-dosage SP group was significantly lower than that in the other three medication groups（P ＜0.05）. Bone mineralization deposition rate in sham operation group and model group did not differ from that in the four medication groups（P \> 0.05）. When compared with the model group，the medication groups had high trabecular bone area percentage（% TbAr），trabecular bone thickness（TbTh），trabecular bone number（TbN），bone formation rate of bone surface，and bone formation rate of bone volume， but had low trabecular bone resolution and osteoclast number（P ＜0.05）. Serum estrogen level was closely correlated with most of bone histomorphometry parameters. Conclusion The estrogen level shows significant correlation with most of bone histomorphometry parameters. Application of middle- and high-dosage SP could improve estrogen level，enhance bone microstructure，inhibit bone structure degradation，promote bone formation and restrain bone resorption.

R285.5

广东省中医药局科研课题（20132119）；教育部高等学校博士点基金（20114425120016）。