目的 探讨传统中药蟾酥逆转人白血病K562/DOX细胞多药耐药性（MDR）的作用。方法 采用中药血清药理学方法制备蟾酥含药血清，以MTT法分别检测蟾酥含药血清联合柔红霉素，或单用蟾酥含药血清处理后，不同时段作用于K562/DOX细胞的抑制率；实时荧光定量PCR法检测蟾酥含药血清对K562/DOX细胞的Bcl-2 mRNA表达。结果 与空白对照组比较，蟾酥对K562/DOX细胞均有抑制作用；蟾酥组Bcl-2 mRNA表达降低。结论 蟾酥可能通过下调MDR1表达水平进而逆转K562/DOX细胞的耐药性。

Objective To explore the effect of Venenum Bufonis on reversing the multidrug resistance of human leukemia cell line K562/DOX. Methods Serum containing Venenum Bufonis was prepared firstly，and then was applied alone or together with Daunorubicin for the culture of human leukemia cell line K562/DOX. After treatment for some time，the inhibition ratio of K562/DOX was detected by MTT assay，and Bcl-2 mRNA expression in K562/DOX was examined by real-time fluorescent quantitation PCR. Results In comparison with the control group，Venenum Bufonis had an inhibition on K562/DOX and decreased the Bcl-2 mRNA expression. Conclusion Venenum Bufonis can down-regulate the MDR1 expression level，and thus reverse the multidrug resistance of human leukemia cell line K562/DOX.

R285.5

广东省中医药管理局资助项目（20121216）；广东省科技发展计划资助项目（2012b03180019）。