目的 观察益气温阳活血法对心力衰竭大鼠心肌细胞线粒体通透性转变的作用。方法 将SD大鼠随机分为对照组、模型组，心阳片低、中、高剂量组，每组6只。除对照组开腹后不行腹主动脉缩窄术，其余大鼠采用腹主动脉缩窄术复制心力衰竭模型，心阳片低、中、高剂量组分别给予心阳片140，281， 562 mg·kg-1，连续2周。采用心脏彩超检测各组大鼠心功能，观察各组心肌细胞线粒体在CaCl2作用15 min后，540 nm吸光值的变化值检测各组心肌细胞线粒体通透性（MPT）转变。通过萤光素-萤光素酶的方法检测心肌细胞三磷酸腺苷（ATP）的含量。结果 模型组左室射血分数（LVEF）和左室短轴缩短率（LVFS）较对照组显著降低（P＜0.01）；心阳片低、中、高剂量组LVEF和LVFS较模型组均显著增加（P＜0.01）。模型组心肌细胞MPT较对照组显著增加（P＜0.01），而心阳片中、高剂量组MPT较模型组显著减少（P＜0.01）。模型组心肌细胞ATP含量较对照组显著减少（P＜0.01），而心阳片低、中、高剂量组ATP含量较模型组均显著增加（P＜0.01）。结论 益气温阳活血法代表方心阳片具有抑制心衰大鼠心肌细胞线粒体通透性转变，改善心功能的作用。

Objective To investigate the effects of therapy of benefiting qi，warming yang and activating blood on mitochondrial permeability transition in cardiomyocytes of heart failure（HF） rats. Methods Thirty SD rats were evenly randomized into five groups，blank control group，heart failure group，and low-，middle- and high-dosage Xinyang tablet（XT） groups. Rats except for the blank control group were given abdominal aorta constriction to induce heart failure，and then were fed orally with normal saline or ST at the dosage of 140，281，562 mg·kg-1 respectively for 2 continuous weeks according to the experimental design. The rats of the blank control group only had the belly cut open. We examined the heart function of rats in three groups by color ultrasonography，analyzed the mitochondrial permeability transition（MPT） by observing the optical absorption value at 540 nm after myocardial cell mitochondria were cocultured with CaCl2 for 15 min，and detected the intracellular ATP content in the cardiomyocytes of HF rats by luciferin- luciferase method. Results Heart failure group had lower left ventricular ejection fraction（LVEF） and left ventricular fractional shortening（LVFS）than the blank control group（P＜0.01），while XT groups had higher LVEF and LVFS than the heart failure group（P＜0.01）. MPT was increased in heart failure group（P＜0.01 compared with that in the blank control group），and then was decreased by middle- and high-dosage XT（P＜0.01 compared with that in the heart failure group）. ATP content was decreased in heart failure group（P＜0.01 compared with that in the blank control group），and then was increased by low-，middle- and high-dosage XT（P＜0.01 compared with that in the heart failure group）. Conclusion Xinyang tablet，a representative prescription of therapy of benefiting qi，warming yang and activating blood，can improve cardiac function through inhibiting the mitochondrial permeability transition in cardiomyocytes of heart failure rats.

R285.5

国家自然科学基金面上项目（81373570）；国家自然科学基金青年科学项目（81102585）；中国博士后科学基金（20110490871）；广东省自然科学基金项目（S2011040003510）。