目的 考察通络醒脑泡腾片对东莨菪碱致拟痴呆小鼠学习记忆及中枢胆碱能神经功能的影响。方法 制备东莨菪碱致拟痴呆小鼠模型，采用避暗法和Morris水迷宫考察通络醒脑泡腾片对小鼠模型学习记忆功能的影响；采用ELISA法检测小鼠脑海马组织乙酰胆碱（Ach）和胆碱乙酰转移酶（ChAT）含量，采用比色法测定AchE含量，考察通络醒脑泡腾片对小鼠模型中枢胆碱能神经功能的影响。结果 避暗测定结果显示通络醒脑泡腾片可以显著延长小鼠的逃避潜伏期，并显著减少错误次数，与模型组比较差异有显著性意义（P＜0.05）；Morris水迷宫检测结果显示通络醒脑泡腾片能够显著缩短小鼠定向航行测试的逃避潜伏期和空间探索测试的第三象限停留时间，显著延长有效区停留时间，增加经过平台次数，与模型组比较差异有统计学意义（P＜0.05）；小鼠脑组织中枢胆碱能神经功能测定结果显示，模型组Ach含量显著降低，ChAT酶活性显著降低，AchE活性显著增高，与空白对照组比较差异有统计学意义（P＜0.05）；与模型组比较，通络醒脑泡腾片高、中、低剂量组Ach活性差异以及中剂量组ChAT活性显著增高，差异有统计学意义（P＜0.05），而通络醒脑泡腾片AchE活性有一定的降低趋势，但差异无统计学意义（P＞0.05）。结论 通络醒脑泡腾片对东莨菪碱所致小鼠拟痴呆模型学习记忆功能具有良好的改善作用，可能与提高模型小鼠中枢胆碱能神经功能、促进乙酰胆碱的合成有关。

Objective To explore the effects of Tongluo Xingnao effervescent tablets on learning and memory and cerebral cholinergic function of dementia mice induced by intraperitoneal injection of scopolamine. Methods Mice model of dementia was established by intraperitoneal injection of scopolamine. The Morris water maze and passive avoidance experiment were adopted to evaluate the learning and memory ability of mice. Enzyme-linked immunosorbent assay（ELISA） was used to measure the contents of acetylcholine（Ach） and acetylcholine transferase（ChAT） in mice hippocampus. Colorimetry was used to measure the activity of acetylcholinesterase（AchE）. And then we evaluated the effects of Tongluo Xingnao effervescent tablets on cerebral cholinergic function of dementia model mice. Results The results of passive avoidance test showed Tongluo Xingnao effervescent tablets could prolong the escape latency time and reduce the frequency of errors，the difference being significant compared with the model control group（P ＜0.05）. The Morris water maze test demonstrated Tongluo Xingnao effervescent tablets could notably shorten the escape latency time of mice in orientated-navigation test，extend retention time in the third quadrant and time in staying active area significantly，and increase the times of crossing platform significantly（P ＜0.05）. The cerebral cholinergic function of brain tissue tests revealed that Ach content and ChAT activity were decreased and AchE activity was increased in the model control group（P ＜0.05 compared with those in the blank control group），and then Ach activity was increased in the three Tongluo Xingnao effervescent tablets groups，ChAT activity was enhanced in middle-dosage Tongluo Xingnao effervescent tablets group（P ＜0.05 compared with those in the model control group）. However，the difference of AchE activity was insignificant（P \> 0.05） between Tongluo Xingnao effervescent tablets group and model control group. Conclusion Tongluo Xingnao effervescent tablets can markedly improve the ability of learning and memory of model mice induced by intraperitoneal injection of Scopolamine，and the mechanism may be associated with improving cerebral cholinergic function and promoting the synthesis of acetylcholine in dementia model mice.

R285.5

重大新药创制科技重大专项（2013ZX09103002-008）；四川省杰出青年学术技术带头人资助计划（2011JQ0014）；四川省学术和技术带头人培养资金资助项目（重点）；四川省教育厅自然科学重点项目（12ZA041）；成都市产业发展技术支撑计划——高校院所应用基础研究（12DXYB321JH-002）。