目的 考察冰片与苯扎氯铵合用对红细胞及角膜上皮细胞的毒性。方法 实验分为溶剂对照组、冰片组、苯扎氯铵组、冰片+苯扎氯铵组，分别测定各组红细胞半数溶血浓度（HC50）和角膜上皮细胞半数抑制浓度（IC50），根据等辐射分析法和响应面分析法判断联合作用类型和趋势，并观察二者合用对眼角膜上皮细胞增殖的影响。结果 苯扎氯铵对红细胞及角膜上皮细胞的毒性作用呈明显的量效关系，加入冰片后能降低其HC50和IC50值。结论 冰片与苯扎氯铵对红细胞的溶血效应表现为协同作用，冰片能增强苯扎氯铵对角膜上皮细胞的毒性作用，受损一定程度内的细胞可恢复。

Objective The in vitro hemolytic test and cytotoxicity test were used to study the toxicity of the combination of borneol and benzalkonium chloride to red blood cells and corneal epithelial cells. Methods Suspension of rabbit red blood cells and corneal epithelial cells were treated with different concentrations of borneol，benzalkonium chloride and combination of borneol-benzalkonium. Dose-response assays were firstly carried out to obtain the 50% hemolysis concentration（HC50） of the individual drug group. Then isobolographic analysis and response surface analysis were used to define the type and tendency of interaction between borneol and benzalkonium chloride. The effects of the combination on the proliferation of corneal epithelial cells were also observed. Results The toxicity of benzalkonium chloride to red blood cells and corneal epithelial cells was dose-dependent. The curve of dose-effect showed that HC50 and IC50 of benzalkonium chloride were decreased after borneol was added. Conclusion Borneol and benzalkonium chloride have synergistic action on the hemolytic effect，and borneol can enhance the toxicity of benzalkonium chloride on corneal epithelial cells. The corneal epithelial cells with certain degrees of damage can recover to be normal.

R285.5

广东省自然科学基金（S2011040003546）；广东省科技计划项目（2011B031700069）。