目的 建立血复生片中芍药苷含量的测定方法。方法 采用高效液相色谱法测定。色谱柱:十八烷基硅烷键合硅胶柱(4.6 mm × 250 mm，5 μm)；流动相:甲醇-0.1 ％磷酸溶液(21 ∶ 79)，检测波长:230 nm，柱温:30 ℃，流速:1.0 mL·min-1。结果 芍药苷进样量在0.066~0.396 μg范围内，进样量与峰面积呈良好的线性关系(r=0.9994)；平均加样回收率为98.72 %，RSD为1.88 %(n=6)。结论 该方法操作简便易行，专属性强，准确可靠，可用于血复生片的质量控制。

Objective To establish a method for the determination of paeoniflorin in Xuefusheng Tablets.Methods HPLC method was carried out on a column of octadecylsilane chemically bonded silica(4.6 mm×250 mm，5 μm) with the mobile phase of methanol -0.1 % phosphoric acid solution(21∶79). Detection wavelength was set at 230 nm，and the column temperature was 30 ℃. The flow rate was 1.0 mL·min-1. Result The injection volume of paeoniflorin and peak area showed a good linear relationship(r=0.9994) in the range of 0.066~0.396 μg. The average recovery was 98.72 %，and RSD was 1.88 %(n=6). Conclusion The method is simple，specific，accurate and reliable，can be used for the quality control of the Xuefusheng Tablets.

重大新药创制科技重大专项,创新药物孵化(吉林)基地建设(2011ZX09401-305)。