[关键词]
[摘要]
目的 考察不同产地蜂胶提取物样品的红外指纹图谱差异,构建蜂胶提取物的红外指纹图谱。方法 采用傅里叶变换红外光谱(FTIR)建立不同产地蜂胶提取物红外指纹图谱,应用OMNIC软件对数据进行预处理,Matlab7.0比较各样品的相似度,SPSS17.0统计软件进行聚类分析。结果 FTIR图谱在690~1760 cm-1波数范围内吸收峰差异较大,可作为蜂胶提取物的指纹特征,红外指纹图谱结合聚类分析的方法,可作为蜂胶快速鉴别的方法。结论 该方法简便、稳定,可用于蜂胶的鉴别和质量评价。
[Key word]
[Abstract]
Objective To investigate the infrared fingerprint differences in propolis extract samples from different areas,and to construct the infrared fingerprint of propolis extract. Methods Fourier transform infrared spectroscopy(FTIR) was adpoted to construct the infrared fingerprint of propolis extract fromvdifferent origins. OMNIC software was applied for data pre-processing,Matlab7.0 for comparing the similarity of each sample,and SPSS17.0 statistical software for cluster analysis. Results The absorption peak varied greatly in the FTIR spectra range of 690~1760 cm-1,which can be used as the specific fingerprint of propolis extract. Cluster analysis method for infrared fingerprint can be used for the rapid identification of propolis. Conclusion The established method is simple and stable,and can be used for identification and quality evaluation of propolis.
[中图分类号]
[基金项目]
广东省教育部产学研结合项目(2011B090400124)。