目的 研究温阳药对糖尿病前期大鼠的降糖作用机制。方法 制备高糖高脂饮食诱导糖尿病前期肥胖大鼠模型，动物随机分为8组:正常对照组、模型组、阳性对照药(二甲双胍)组、阴性复方组(无温阳及补肾药对)、补肾阳药对组、补肾阳复方组、温阳药对组、温阳复方组。模型复制后开始给药，每天1次，连续8周。实验结束后采用Western bolt法检测肝脏葡萄糖转运蛋白2(GLUT2)、肌肉葡萄糖转运蛋白4(GLUT4)的表达水平。结果 与模型组比较，温阳复方组能减轻大鼠体质量、降低肥胖指数(Lee’s Index)、抑制血糖升高(P＜0.01，P＜0.05)，还能明显上调大鼠肝脏GLUT2及骨骼肌GLUT4的表达水平(P＜0.01)。结论 温阳药的降糖作用可能与改善GLUT2及GLUT4的表达抑制有关。

Objective To study the possible hypoglycemic mechanism of yang-warming Chinese medicine in prediabetic rats. Methods High fat and sugar diet was given to the rats to induce prediabetes-obesity rat model，and then the model rats were randomly divided into 8 groups，namely normal control group，model group，metformin group，negative compound group，kidney-yang-tonifying drug pair group，kidney-yang-tonifying compound recipe group，yang-warming drug pair group，and yang-warming compound recipe group. Meanwhile，a normal control group was set up. Medication started after modeling，once a day，lasting for 8 weeks. Expression levels of hepatic glucose transporter 2(GLUT2) and muscular glucose transporter 4(GLUT4) were measured by Western blotting. Results The body mass of rats and Lee's index in the model group were significantly higher than those of the normal control group(P＜0.01). Compared with the model group，rat body mass and Lee 's index were decreased，the increased blood glucose was suppressed(P＜0.01，P＜0.05)，and the expression levels of GLUT2 and GlUT4 were upregulated in yang-warming compound recipe group(P＜0.01). Conclusion The possible hypoglycemic mechanism of yang-warming Chinese medicine in prediabetic rats is related with the upregulation of expressions of GLUT2 and GLUT4.

广州中医药大学(东莞)中医药数理工程研究院首期支撑项目(2009CCQ1001)；广东省科技计划项目(2011A030100016)；广东省教育部产学研结合项目(2012B091100184)。