目的 探讨白藜芦醇诱导鼻咽癌CNE-1细胞凋亡及其分子机制。方法 噻唑蓝(MTT)比色法检测细胞活力；活细胞染色法(PI)流式细胞仪分析细胞周期和PI-AnnexinV双染色法流式细胞仪检测细胞凋亡；免疫印迹检测蛋白表达水平。结果 MTT结果表明白藜芦醇呈浓度和时间依赖性抑制鼻咽癌CNE-1细胞活力，与正常对照组比较，差异有统计学意义(P < 0.01)；PI单染流式细胞仪分析表明白藜芦醇引起S期的CNE-1比例明显增多，相应免疫印迹结果显示细胞周期蛋白Cyclin E和Cyclin A显著下调，呈药物浓度依赖；PI-Annexin V双染流式细胞仪分析表明白藜芦醇呈药物浓度依赖诱导CNE-1细胞凋亡；免疫印迹分析显示抗凋亡蛋白Bcl-2表达下调，而促凋亡蛋白Bax表达上调。结论 白藜芦醇能抑制鼻咽癌CNE-1细胞的增殖，使其阻滞在S期，并可能通过线粒体介导的凋亡通路诱导其凋亡。

Objective To elucidate the effects of resveratrol on inducing apoptosis of nasopharyngeal carcinoma CNE-1 cells and its molecular mechanism. Methods The viability of CNE-1 cells was detected by methyl thiazolyl tetrazolium(MTT) assay，cell cycle profile was analyzed by PI single-staining flow cytometer and apoptosis of CNE-1 cells was analyzed by PI-AnnexinV double-staining flow cytometer. The expression level of target proteins was examined by immunoblotting method. Results Resveratrol significantly inhibited the viability of CNE-1 cells in concentration- and time-dependent manner(P < 0.01 compared with the normal control group). Treatment with resveratrol resulted in the increased the percentage of S-phase CNE-1 cells in dose-dependent manner. Accordingly，Cyclin E and Cyclin A were downregulated in resveratrol-treated CNE-1 cells. Furthermore，resveratrol dose-dependently induced the apoptosis of CNE-1 cells. Correspondingly，the anti-apoptotic protein Bcl-2 was downregulated and the pro-apoptotic protein Bax was upregulated in CNE-1 cells after treatment with resveratrol. Conclusion Resveratrol can arrest CNE-1 cells at S phase via downregulating the expression of cyclinE and Cyclin A，and can further induce apoptosis through mitochondrial-mediated intrinsic cell-death pathways.

国家自然科学基金(81274145)；广东省中医药局科研基金(20121227)；教育部高等学校博士学科点专项科研基金(20124425120012)。