目的 采用体外炎症模型观察小叶榕叶水提物及其不同极性萃取部位对炎症介质一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)的影响。方法 采用脂多糖(LPS)刺激RAW264.7巨噬细胞，建立体外炎症模型。采用噻唑蓝(MTT)法检测小叶榕叶水提物及其不同萃取部位对RAW264.7巨噬细胞的毒性作用；Griess法检测培养液中NO含量；采用酶联免疫试验(ELISA)法检测培养液中TNF-α的含量。结果 LPS刺激RAW264.7巨噬细胞后，培养液中NO和TNF-α含量显著增加(P < 0.01)；小叶榕叶水提物及其不同萃取部位对NO和TNF-α的释放有不同程度的抑制作用，其中水提物高、中、低浓度组对NO的抑制率分别为17.0 %，30.0 %，33.0 %；乙酸乙酯部位中、低浓度组对NO的抑制率分别为40.0 %、27.0 %；水相部分低浓度组对NO的抑制率为37.0 %；水提物中浓度组和乙酸乙酯部位高浓度组对TNF-α的抑制率分别为58.0 %，43.5 %，与LPS组比较，差异均有统计学意义(P < 0.01)。结论 小叶榕叶可能通过抑制NO和TNF-α释放而发挥抗炎作用，其中乙酸乙酯部位、水相部分是小叶榕叶的主要抗炎活性成分。

Objective To evaluate the effects of water extract of Ficus microcarpa leaves and the polar solvent extract of different parts on the release of inflammatory mediators of nitric oxide(NO) and tumor necrosis factor alpha(TNF-α) by using in-vitro inflammatory model. Methods Lipopolysaccharide(LPS) was used to stimulate the RAW264.7 macrophage line for the establishment of inflammatory model. MTT assay was performed to determine the toxic effects of water extract of Ficus microcarpa leaves and the polar solvent extract of different parts on the viability of RAW264.7. Griess reaction was used for assaying the concentration of NO，and ELISA was performed to measure the expression of TNF-α in the cultured solution. Results NO and TNF-α contents in the cultured solution of macrophages were significantly increased after the stimulation of LPS(P < 0.01). Water extract of Ficus microcarpa leaves and the polar solvent extract of different parts could remarkable ameliorate the inflammation factors release at various degrees. The inhibition of NO was 17.0 %，30.0 %，33.0 % in the high-，middle- and low-concentration water extract groups，respectively. The inhibition of NO was 40.0 %，27.0 % in the middle- and low-concentration acetoacetate fraction groups，respectively，and was 37.0% in the low-concentration water fraction group. Middle-concentration water extract and high-concentration acetoacetate fraction presented the inhibition of TNF-α being 58.0 %，43.5 %，respectively，and the differences were significant when compared with LPS group(P < 0.01). Conclusion Ficus microcarpa leaves exert anti-inflammatory action by inhibiting the release of NO and TNF-α，and the acetoacetate fraction and water fraction are the main anti-inflammatory active constitutents of Ficus microcarpa leaves.

教育部留学回国人员科研启动基金([2011]1139)；国家自然科学基金青年基金资助项目(30801515)。