目的 研究黄芩素对子宫颈癌Hela细胞表皮生长因子受体(EGFR)表达的影响，探讨黄芩素抗肿瘤的分子机制。方法 采用CCK-8法检测黄芩素对Hela细胞增殖的抑制作用，real-time PCR法和Western blot法检测黄芩素对Hela细胞EGFR表达的影响。结果 黄芩素可在体外抑制Hela细胞的增殖，并对EGFR的表达有显著的抑制作用，呈剂量依赖性。结论 黄芩素可能是通过EGFR通路抑制子宫颈癌Hela细胞的增殖。

Objective To investigate the effect of baicalein on the expression of human cervix cancer Hela cells epidermal growth factor receptor(EGFR)，thus to explore its anti-tumor molecular mechanism. Methods Anti- proliferative effects of baicalein on the proliferation of human cervix cancer Hela cells were assessed by using CCK-8 assay. The effects of baicalein on the regulation of EGCG mRNA and protein expression were examined by using real-time PCR and Western blotting analysis. Results The results of CCK-8 assay indicated that baicalein markedly decreased the viability of Hela cells compared to control groups. Furthermore，the levels of EGCG mRNA and protein expression were suppressed significantly by baicalein treatment in dose-dependent manner. Conclusion These findings demonstrate that the ability of baicalein in inhibiting the proliferation of human cervix cancer Hela cells may be associated with EGFR pathway.