目的 探讨建立乙醇诱导人胃上皮细胞株(GES-1)凋亡模型的方法和可行性。方法 采用罗氏细胞实时动态分析仪、MTT比色法、Hoechst染色法、Annexin V-FITC/PI凋亡流式检测研究不同浓度的乙醇对人胃上皮细胞株(GES-1)凋亡作用。结果 (1)当乙醇浓度大于0.5 mol·L-1时，对GES-1细胞株有明显的诱导凋亡作用，而考察的TNF-α(100，50 ng·mL-1)则没有明显的细胞凋亡诱导作用；(2)通过形态学观察，发现乙醇模型组GES-1细胞株出现明显的凋亡细胞形态学改变；(3)Annexin V-FITC/PI凋亡流式分析结果发现乙醇浓度大于0.2 mol·L-1对细胞具有明显的诱导凋亡作用，乙醇主要诱导GES-1细胞株进入早期凋亡，且具有浓度依赖性，但该诱导作用具有时效性，在作用持续60 min后，其诱导作用会逐渐减弱。结论 乙醇对GES-1细胞株具有明显的诱导细胞凋亡作用，且具有浓度依赖性及时效性；本凋亡模型具有较好的重现性。

Objective To explore the feasibility of establishing the model of human gastric epithelial cells(GES-1)apoptosis model induced by ethanol. Methods Cell viability and apoptosis induced by ethanol were assessed by MTT assay，cell real-time dynamic analyzer，Hoechst staining，and Annexin V-FITC/PI. Results (1)When the concentration of ethanol in cell culture medium was higher than 0.5 mol·L-1，GES-1 showed obvious injury and apoptosis. TNF-α (100，50 ng·mL-1)showed no obvious apoptosis induction for the GES-1. (2)The morphological examination showed that the GES-1 of ethanol group appeared apoptotic morphological changes. (3)The results of Annexin V - FITC/PI combined with flow cytometry analysis showed that ethanol at the concentration higher than 0.2 mol·L-1 induced the apoptosis of GES-1，and the apoptotic cells were almost in early-stage apoptosis，the effect being concentration-dependent. But after 60 minutes，the effect would gradually weaken. Conclusion The results demonstrated that ethanol can induce the apoptosis of GES-1 in time- and dose-dependent. The established apoptosis model has good reproducibility.

R285.5

国家自然科学基金资助项目(81072946)。