目的 观察aP2-SREBP-1c转基因小鼠糖脂代谢生物特征，探讨其在糖脂代谢紊乱性疾病研究中的应用。方法 动物分为aP2-SREBP-1c转基因小鼠及其同窝野生型小鼠2组，均为雌性，观察两组小鼠体质量、进食量、进水量，不禁食及禁食12 h血中TG、TC、LDL-C、Glu水平，肝脏、肌肉脂质含量，口服糖耐量、胰岛素及瘦素，稳态胰岛素(HOMA-IR)，脂肪、肝脏病理组织学变化。结果 与野生型比较，aP2-SREBP-1c转基因小鼠肾周、性腺周围、皮下脂肪均明显减少(P＜0.01)，褐色脂肪明显增加(P＜0.01)，肝脏切片显示明显脂肪肝病理变化，肝脏质量明显增加(P＜0.01)，血中胰岛素、葡萄糖水平及HOMA-IR明显增加(P分别＜0.01、0.05、0.001)，瘦素明显下降(P＜0.01)，禁食状态血中TC、LDL-C水平均显著升高(P < 0.05，P ＜0.01)，显示存在明显的脂肪分化障碍、胰岛素抵抗、高胆固醇血症和脂肪肝。结论 aP2-SREBP-1c转基因小鼠可用于糖尿病、脂肪肝、高胆固醇血症等糖脂代谢紊乱疾病的研究。

Objective To observe the biometric characteristics of glucose and lipid metabolism of aP2-SREBP-1c transgenic mice，and to explore its application in the study of glucose and lipid metabolism disorder. Methods Female aP2-SREBP-1c transgenic mice and their wild type littermates were investigated. General data of body weight，food-intake and drink-intake were monitored. Metabolic parameters such as fed and fasting plasma glucose，TG，TC，LDL-C，hepatic TC，TG and muscular TG， fasting blood glucose(FBG)，insulin，leptin， HOMA-IR were tested. Histological changes in fat tissue and hepatic tissue were observed after hematoxylin and eosin staining. Results Compared to their wild type littermates，weight of perirenal fat，peri-gonad fat，subcutaneous fat lose in the transgenic mice(P < 0.01)，while weight of brown adipose tissue and liver gained(P < 0.01). Liver biopsy showed significantly pathological changes of fatty liver. Plasma insulin，glucose and HOMA-IR raised significantly(P < 0.01，P < 0.05，P < 0.001)，while plasma leptin level decreased(P < 0.01). Lipid metabolic indicators such as fasting plasma TC and LDL-C were higher(P < 0.05，P < 0.01). The results indicated that aP2-SREBP-1c mice were characterized by evident lipodystrophy，insulin resistance，hypercholesteremia and fatty liver when compared with the wild-type controlled group. Conclusion aP2-SREBP-1c transgenic mice can be used as the advisable mouse model for the study of glucose and lipid metabolism disorder such as insulin resistance，fatty liver and hypercholesteremia.

R285.5

教育部“新世纪优秀人才计划”(NCET-11-0916)；国家自然科学基金面上项目(81274156)；广州市科技攻关专项(2012J4100095)。