目的 建立测定菟丝子中绿原酸、金丝桃苷、异槲皮苷、紫云英苷、山奈酚的含量方法。方法 采用高效液相色谱(HPLC)法。色谱柱为Zorbax SB C18；流动相为乙腈-0.1 %磷酸溶液，梯度洗脱；绿原酸的检测波长为327 nm，金丝桃苷、异槲皮苷、紫云英苷、山奈酚的检测波长为360 nm；流速为1.0 mL·min-1；柱温为30 ℃。结果 5种活性成分均达到基线分离，线性关系良好(r≥0.9998)；平均加样回收率为96.8 %～101.0 %，RSD＜3.0 %(n=6)。结论 该方法灵敏，可靠，可用于菟丝子药材的质量评价。

Objective To establish a method for the content determination of chlorogenic acid，hyperoside，isoquercitrin，astragalin and kaempferol in Semen Cuscutae by high performance liquid chromatography(HPLC). Methods HPLC was performed on Zorbax SB C18 column，the acetonitrile-0.1 % phosphoric acid(gradient elution)was employed as the mobile phase，and detection wavelength was 327 nm for chlorogenic acid and 360 nm for hyperoside，isoquercitrin，astragalin and kaempferol. Column temperature was 30 ℃，and flow rate was 1.0 mL·min-1. Results Baseline separation were obtained and linear relationship were good(r≥0.9998) for the five main constitutes. The average recoveries were 96.8 %～101.0 % and RSD ＜ 3.0 %(n=6). Conclusion The established method is sensitive，reliable and available for the quality control of Semen Cuscutae.

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